藏药俄色改善KK-Ay小鼠脂代谢紊乱的作用研究  

Effects of Tibetan Medicine Ese in Treatment of Lipid Metabolism Disorders in KK-Ay Mice

作　　者：华桦[1] 刘俐 刘芳[2] 朱宁[1] 赵军宁[1] Hua Hua;Liu Li;Liu Fang;Zhu Ning;Zhao Junning(Sichuan Institute for Translational Chinese Medicine,Translational Chinese Medicine Key Laboratory of Sichuan Province,Biological Assay Key Laboratory of State Administration of Traditional Chinese Medicine for Traditional Chinese Medicine Quality,Engineering Research Center for Formation Principle and Quality Evaluation of Genuine Medicinal Materials in Sichuan Province,Sichuan Engineering Technology Research Center of Genuine Regional Drug,Sichuan Academy of Chinese Medicine Sciences,Chengdu 610041;West China School of Pharmacy,Sichuan University,Chengdu 610041)

机构地区：[1]四川省中医药科学院四川省中医药转化医学中心,中医药转化医学四川省重点实验室,国家中医药管理局中药质量生物评价重点研究室,四川省道地药材形成原理与品质评价工程研究中心,四川省道地药材系统开发工程技术研究中心,成都610041 [2]四川大学华西药学院,成都610041

出　　处：《中药药理与临床》2022年第2期141-146,共6页Pharmacology and Clinics of Chinese Materia Medica

基　　金：国家自然科学基金青年科学基金项目(编号:81703820)

摘　　要：目的:研究藏药俄色改善KK-Ay小鼠脂代谢紊乱的作用及可能的作用机制。方法:10只C57BL/6J小鼠作为正常对照组,50只KK-Ay模型小鼠按空腹血糖值随机分为模型对照组、阳性对照组(罗格列酮片2.67 mg/kg)和俄色提取物0.75、1.5、3.0 g原生药/kg组,每组10只,连续灌胃给药35 d,末次药后测定小鼠血清游离脂肪酸(FFA)、三酰甘油(TG)、总胆固醇(TC)和肿瘤坏死因子-α(TNF-α)含量;油红O染色法检测肝脏组织中脂质的含量;免疫组化法检测肾周脂肪组织中超氧化物酶体增殖物激活受体-α(PPAR-α)和PPAR-γ蛋白表达;实时定量荧光(PCR)检测Ppar、Tnfa mRNA表达。结果:与正常对照组比较,模型对照组小鼠体质量显著升高,血清FFA、TG、TC、TNF-α含量显著升高(P<0.01),小鼠皮下脂肪系数和腹腔脂肪系数显著升高,小鼠肝脏组织脂滴面积百分比显著升高(P<0.01),小鼠脂肪组织PPAR-α、γ蛋白表达明显降低,肾周脂肪组织Ppar mRNA表达明显下调(P<0.05);与模型对照组比较,俄色提取物1.5、3.0 g/kg组小鼠体质量和皮下脂肪系数明显降低,小鼠肝脏组织脂滴面积百分率明显降低,脂肪组织PPAR-α蛋白明显升高,肾周脂肪组织Ppar mRNA表达明显上调(P<0.05或P<0.01);俄色提取物3.0 g/kg组小鼠血清FFA、TG、TNF-α含量明显降低,肾周脂肪组织Tnfa mRNA表达明显降低(P<0.05或P<0.01);俄色提取物0.75 g/kg组小鼠血清TC含量明显降低、小鼠皮下脂肪系数降低(P<0.05)。结论:藏药俄色可改善KK-Ay小鼠脂质代谢紊乱,其作用机制可能与上调肾周脂肪组织PPARα蛋白表达和PPARγ转录以及减轻炎症反应有关。Objective:To investigate the effects and underlying mechanism of Tibetan medicine Ese on lipid metabolism disorders in KK-Ay mice.Methods:Ten C57 BL/6 J mice were assigned to a normal control group.Fifty KK-Ay model mice were randomly divided into a model control group,a rosiglitazone(2.67 mg/kg)group,and low-,medium-,and high-dose Ese extract groups(0.75 g,1.5 g,and 3.0 g/kg crude drug),with 10 mice in each group according to fasting blood glucose levels.The serum levels of free fatty acid(FFA),triglyceride(TG),total cholesterol(TC),and tumor necrosis factor-α(TNF-α)were determined after 35 days of continuous administration by gavage.Lipid content in liver tissues was detected by oil red O staining.The protein expression of peroxisome proliferator-activated receptor-α(PPAR-α)and peroxisome proliferator-activated receptor-γ(PPAR-γ)in perirenal adipose tissues was determined by the immunohistochemical method.The mRNA expression of PPAR and TNF-αwas detected by real-time quantitative PCR.Results:Compared with the normal control group,the model control group showed increased body weight,increased serum content of FFA,TG,TC,and TNF-α(P<0.01),elevated subcutaneous fat and abdominal fat coefficients,increased percentage of lipid droplet expression area in liver tissues(P<0.01),reduced protein expression of PPAR-αand PPAR-γin adipose tissues,and down-regulated PPAR mRNA expression in perirenal adipose tissues(P<0.05).Compared with the model control group,the medium-and high-dose Ese extract groups displayed reduced body weight and subcutaneous fat coefficient(P<0.05),decreased percentage of lipid droplet expression area in liver tissues,increased protein expression of PPAR-αin adipose tissues,and up-regulated expression of PPAR mRNA in perirenal adipose tissues(P<0.01 or P<0.05).In the high-dose Ese extract group,the serum levels of FFA,TG,and TNF-αdecreased and the expression of TNF-αmRNA in perirenal adipose tissues was reduced(P<0.01 or P<0.05).In the low-dose Ese extract group,the serum TC level was red

关 键 词：俄色 自发性2型糖尿病小鼠 脂代谢 超氧化物酶体增殖物激活受体-γ 肿瘤坏死因子-Α 

分 类 号：R29[医药卫生—民族医学]