HPLC法同时测定狭叶竹节参中4种皂苷成分的含量  

Simultaneous Determination of Four Saponins in Panax Japonicus var. Angustifolius by HPLC

作　　者：姜薇薇 陈庚[2,3] 沈雁南 唐卿燕 唐军荣 卢迎春 Jiang Weiwei;Chen Geng;Shen Yannan;Tang Qingyan;Tang Junrong;Lu Yingchun(College of Science,Yunnan Agricultural University,Kunming 650201,China;Key Laboratory of Medicinal Plant Biology of Yunnan Province,Yunnan Agricultural University,Kunming 650201,China;National&Local Joint Engineering Research Center on Germplasms Utilization&Innovation of Chinese Medicinal Materials in Southwest,Yunnan Agricultural University,Kunming 650201,China)

机构地区：[1]云南农业大学理学院 [2]云南农业大学云南省药用植物生物学重点实验室 [3]云南农业大学西南中药材种质创新与利用国家地方联合工程研究中心,昆明650201

出　　处：《中国药师》2020年第3期585-587,共3页China Pharmacist

基　　金：国家重点研发计划项目(编号:2017YFC1702500);国家自然科学基金联合基金项目(编号:U1402262);云南省教育厅科学研究基金项目(编号:2017ZZX037);云南省农业联合面上项目(编号:2018FG001-037).

摘　　要：目的:建立同时测定狭叶竹节参中人参皂苷Rb1、人参皂苷Ro、竹节参皂苷Ⅳ和竹节参皂苷Ⅳa4种皂苷成分含量的HPLC法。方法:采用Phenomenex Kinetex Biphenyl(250 mm×4.6 mm,5μm)色谱柱流动相为乙腈(A)-0.2%磷酸溶液(B),梯度洗脱流速:1.0 ml·min-1,检测波长:203 nm,柱温:30℃。结果:人参皂苷Rb1、人参皂苷Ro、竹节参皂苷Ⅳ和竹节参皂苷Ⅳa分别在0.20~4.02μg、1.84~36.80μg、0.49~9.80μg和0.40~8.04μg范围内呈良好线性关系(r≥0.9990),平均回收率分别为102.20%,100.77%,100.51%,102.72%,RSD分别为1.71%,1.02%,1.38%,2.39%(n=9)。结论:该方法简便、准确,分离效果好,可为狭叶竹节参药材的质量控制提供参考。Objective:To establish an HPLC method for the simultaneous determination of ginsenoside Rb1,ginsenoside Ro,Chikusetsu saponinⅣand Chikusetsu saponinⅣa in the roots of Panax japonicus var.angustifolius.Methods:The four constituents were determined on a Phenomenex Kinetex Biphenyl column(250 mm×4.6 mm,5μm)with gradient elution by acetonitrile-0.2%phosphoric acid solution at the detection wavelength of 203 nm and a flow rate of 1.0 ml·min-1.The column temperature was 30℃.Results:The linearity of each standard was established within the concentration range of 0.20-4.02μg,1.84-36.80μg,0.49-9.80μg and 0.40-8.04μg with r≥0.9990,respectively.The average recoveries were 102.20%,100.77%,100.51%and 102.72%,and RSDs were 1.71%,1.02%,1.38%and 2.39%(n=9).Conclusion:The developed method is simple,accurate,well separated and precise,which can be used as the quantitative analysis method for Panax japonicus var.angustifolius.

关 键 词：狭叶竹节参 高效液相色谱法 人参皂苷RB1 人参皂苷Ro 竹节参皂苷Ⅳ 竹节参皂苷Ⅳa 

分 类 号：R284.1[医药卫生—中药学] O657.72[医药卫生—中医学]