长叶薄荷酮对耐药大肠埃希菌生物被膜及其调控基因LuxS的影响  

Effect of(R)-(+)-Pulegone on Biofilm Formation and Regulation Gene LuxS of Drug-Resistant Escherichia Coli

作　　者：宫海燕[1] 赵智龙 熊文娟 周晓英[2] GONG Haiyan;ZHAO Zhilong;XIONG Wenjuan;ZHOU Xiaoying(The Fifth Hospital of Xinjiang Medical University,Urumqi 830011,Xinjiang,China;College of Pharmacy,Xinjiang Medical University,Urumqi 830011,Xinjiang,China)

机构地区：[1]新疆医科大学第五附属医院,新疆乌鲁木齐830011 [2]新疆医科大学药学院,新疆乌鲁木齐830011

出　　处：《中华中医药学刊》2022年第3期33-36,共4页Chinese Archives of Traditional Chinese Medicine

基　　金：国家自然科学基金(82060748);新疆自然科学基金面上项目(2015211C025);新疆维吾尔自治区卫生健康青年医学科技人才专项科研项目(WJWY-201916)

摘　　要：目的研究长叶薄荷酮对耐药大肠埃希菌生物被膜形成的抑制作用及其主要调控基因S-核糖基高半胱氨酸酶(S-ribosyl homocysteine lyase,LuxS)的影响。方法采用微量稀释和平板菌落计数法,检测长叶薄荷酮对耐药大肠埃希菌的体外抑菌活性。应用TTC和结晶紫半定量法检测长叶薄荷酮对耐药大肠埃希菌生物被膜形成的抑制作用。RT-PCR方法检测在长叶薄荷酮作用下,耐药大肠埃希菌生物被膜主要调控基因LuxS的变化。结果长叶薄荷酮对耐药大肠埃希菌的生长具有明显的抑制作用,其MIC和MBC分别是23.68、47.35 mg/mL;对其生物被膜形成的抑制作用较强,在94.70 mg/mL浓度下抑制率达到了52.36%,同时长叶薄荷酮明显抑制LuxS的表达(P<0.01)。结论长叶薄荷酮抑制耐药大肠埃希菌的生长和生物被膜的形成,下调LuxS的表达,这为进一步探索长叶薄荷酮抗生物被膜形成的机制奠定分子学基础。Objective To study the inhibition effect of(R)-(+)-pulegone on the biofilm formation and influence on key regulation gene LuxS of drug-resistant Escherichia Coli.Methods The microdilution and plate colony counting methods were used to determine in vitro antibacterial activity of(R)-(+)-pulegone to drug-resistant E.coli.The methods of TTC and semi-quantitative detection of crystal violet were used to detect antibiofilm formation action of(R)-(+)-pulegone to drug-resistant E.coli.With the method of RT-PCR,the change of gene LuxS from drug-resistant E.coli.under the influence of(R)-(+)-pulegone was studied.Results The drug-resistant E.coli was significantly inhibited by(R)-(+)-pulegone and the values of MIC and MBC were 23.68 mg/mL and 47.35 mg/mL,respectively.Meanwhile,the(R)-(+)-pulegone showed strong inhibition activity,and the inhibition rate was 52.36%with the concentration of 94.70 mg/mL.At the same time,(R)-(+)-pulegone significantly inhibited the expression of LuxS(P<0.01).Conclusions(R)-(+)-pulegone could inhibit growth and biofilm formation of drug-resistant E.coli,which established the molecular foundation for further exploration of the anti-biofilm formation mechanism of(R)-(+)-pulegone.

关 键 词：长叶薄荷酮 大肠埃希菌 生物被膜 LUXS 

分 类 号：R285[医药卫生—中药学]