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作 者:徐子龙 张福斌 武兆辰 张晶 张建珍[2] 赵小明[2] XU Zilong;ZHANG Fubin;WU Zhaochen;ZHANG Jing;ZHANG Jianzhen;ZHAO Xiaoming(School of Life Sciences,Shanxi University,Taiyuan 030006,China;Institute of Applied Biology,Shanxi University,Taiyuan 030006,China)
机构地区:[1]山西大学生命科学学院,太原030006 [2]山西大学应用生物学研究所,太原030006
出 处:《中国生物防治学报》2022年第4期1001-1008,共8页Chinese Journal of Biological Control
基 金:山西大学第十八期本科生科研训练项目资助(2020018381);国家自然科学基金(31970469,31702067)
摘 要:Notch是Notch信号通路中的受体蛋白,与配体结合后调控下游靶标基因的表达,在无脊椎动物和脊椎动物的发育和分化过程中发挥着重要的作用。本文以飞蝗为研究对象,利用转录组数据库搜索获得Notch信号通路受体基因LmNotch,并利用qRT-PCR对5龄飞蝗不同组织及不同发育时期翅芽组织中的LmNotch表达特性进行了分析。通过RNA干扰试验对其生物学功能进行分析,发现注射ds LmNotch的飞蝗有70%无法完成蜕皮过程,10%的飞蝗在蜕皮后出现翅紊乱表型。飞蝗翅芽石蜡切片的H&E染色结果表明,处理组翅上下两层表皮之间腔空间增大,新表皮无法完整形成。qRT-PCR检测其他翅发育相关基因表达发现,LmNotch表达量的下降影响翅发育相关基因Dpp、Omb、Yorkie以及翅特异表皮蛋白基因的表达。上述研究结果可为昆虫翅发育机制研究提供理论基础,同时为害虫生物防治提供潜在靶标。Notch is a receptor protein in the Notch signaling pathway,regulating the expression of downstream target genes after binding with ligands and playing an important role in the development and differentiation of invertebrates and vertebrates.In this study,the Notch signaling receptor gene Lm Notch was identified based on transcriptome database of Locusta migratoria,and the expression characteristics of Lm Notch in different tissues and wing pads of different development days of the fifth-instars were analyzed by qRT-PCR.The biological function of LmNotch was analyzed by RNA interference experiment.Compared with the control group,70%of the locusts injected with ds Lm Notch could not complete the molting process,and 10%of the locusts showed wing disorder phenotype after molting.The results of H&E staining on the paraffin section of wing pads showed that,compared with the control group,the cavity space between the upper and lower cuticles of the wing in the treatment group was enlarged,and a complete new cuticle could not be formed.The expression of other wing developing-related genes,such as Dpp,Omb,Yorkie,and the wing specific cuticle protein genes,was affected by the decrease of Lm Notch expression.These results shed light on the mechanism of insect wing development and provide potential targets for pest biological control.
分 类 号:S433.2[农业科学—农业昆虫与害虫防治]
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