莫海威芽胞杆菌D50产抗菌脂肽培养基优化  

Optimization of Medium for the Production of Antifungal Lipopeptides by Bacillus mojavensis Strain D50

作　　者：顾学虎 郑丽宁 黄鑫 翟乾行 张义 张浩 梁爽 GU Xuehu;ZHENG Lining;HUANG Xin;ZHAI Qianhang;ZHANG Yi;ZHANG Hao;LIANG Shuang(College of Plant Protection,Jilin Agricultural University,Changchun 130118,China;College of Resource and Environmental,Jilin Agricultural University,Changchun 130118,China)

机构地区：[1]吉林农业大学植物保护学院,长春130118 [2]吉林农业大学资源与环境学院,长春130118

出　　处：《中国生物防治学报》2022年第4期896-903,共8页Chinese Journal of Biological Control

基　　金：国家重点研发计划(2019YFD1002000)

摘　　要：莫海威芽胞杆菌B.mojavensis D50是一株对多种植物病原真菌具有拮抗作用的生防菌株,前期研究表明脂肽类化合物是其产生的主要抑菌物质。本研究采用抑菌圈法测定了菌株D50抗菌脂肽的稳定性,发现其脂肽粗提液在高温、非强酸强碱、紫外辐射的环境下都具有较高的稳定性。通过Plackett-Burman和Box-Behnken试验,测定了不同培养基成分对脂肽产量的影响,并对菌株D50产抗菌脂肽培养基进行了优化。结果表明,硫酸镁、葡萄糖、酵母浸粉是影响脂肽产量的关键性因素。优化后的培养基成分为葡萄糖15.45 g/L、牛肉膏3.00 g/L、酵母浸粉5.99 g/L、硫酸锰0.30 g/L、氯化钙0.30 g/L、硫酸铵2.00 g/L、硫酸镁0.16 g/L、磷酸二氢钾1.50 g/L和磷酸氢二钠2.00 g/L。培养基优化后,脂肽产量可达1.74 g/L,比优化前提高了167.70%。本研究明确了莫海威芽胞杆菌D50产生的抗菌脂肽对环境因子具有较高的稳定性,并获得了利于抗菌脂肽产生的培养基组分,为D50抗菌脂肽的大规模生产奠定了基础。Bacillus mojavensis strain D50 is a broad-spectrum biocontrol strain with antagonistic effects on many pathogenic fungi.Previous studies showed that the main antifungal substances produced by D50 were lipopeptide compounds.In this study,the stability of antifungal lipopeptide of strain D50 was determined by inhibitive clear zone method.It was found that the crude extract of lipopeptide had high stability in the environment of high temperature,non-extreme acid or alkaline condition and UV radiation.The medium for the production of antifungal lipopeptides from D50 was optimized by Plackett-Burman design and Box-Behnken response surface design methodology.The results showed that magnesium sulfate,glucose and yeast extract powder were the key factors to affect the lipopeptides yield.And the optimized medium compositions were determined as follows:glucose 15.45 g/L,beef extract 3.00 g/L,yeast extract powder 5.99 g/L,manganese sulfate 0.30 g/L,calcium chloride 0.30 g/L,ammonium sulfate 2.00 g/L,magnesium sulfate 0.16 g/L,potassium dihydrogen phosphate 1.50g/L,and disodium hydrogen phosphate 2.00 g/L.By using this medium,the yield of lipopeptides reached 1.74 g/L with a higher proportion of 167.7%than that in the original medium.This study showed that the antifungal lipopeptide produced by strain D50 had high stability to environmental factors.The medium optimization provided valuable information for the large-scale production of antifungal lipopeptide by strain D50.

关 键 词：Bacillus mojavensis D50 抗菌脂肽 培养基优化 脂肽稳定性 

分 类 号：S476.1[农业科学—农业昆虫与害虫防治]