我国葡萄灰霉病菌对四霉素和啶酰菌胺的敏感性测定  

Sensitivity of Botrytis cinerea to Tetramycin and Boscalid in China

作　　者：田秀 周连柱 黄晓庆[2] 孔繁芳[2] 王忠跃[2] 甄志先[1,3] 张昊 TIAN Xiu;ZHOU Lianzhu;HUANG Xiaoqing;KONG Fanfang;WANG Zhongyue;ZHEN Zhixian;ZHANG Hao(College of Forestry,Hebei Agricultural University,Baoding 071000,China;State Key Laboratory for Biology of Plant Diseases and Insect Pest/Institute of Plant Protection,Chinese Academy of Agricultural Sciences,Beijing 100193,China;Key Laboratory of Forest Germplasm Resources and Forest Protection,Baoding 071000,China)

机构地区：[1]河北农业大学林学院,保定071000 [2]中国农业科学院植物保护研究所/植物病虫害生物学国家重点实验室,北京100193 [3]河北省林木种质资源与森林保护重点实验室,保定071000

出　　处：《中国生物防治学报》2022年第4期868-873,共6页Chinese Journal of Biological Control

基　　金：国家重点研发计划(2019YFD1002000)

摘　　要：灰霉病是葡萄生产上的重要病害之一,严重影响葡萄果实的品质和产量。为明确葡萄灰霉病菌对四霉素和啶酰菌胺的敏感性,本研究分别采用菌丝生长速率法和孢子萌发法测定了四霉素和啶酰菌胺对采自云南省宾川县、湖北省武汉市和辽宁省北镇市60株葡萄灰霉病菌的有效抑制中浓度EC,建立了敏感性基线,并分析了二者之间的交互抗性。结果显示,葡萄灰霉病菌对四霉素和啶酰菌胺的敏感基线分别为0.245和1.115μg/mL;上述葡萄灰霉病菌均对四霉素敏感,而11.7%的菌株表现为啶酰菌胺抗性。并且四霉素与啶酰菌胺之间不存在交互抗性(r=-0.040,P>0.05)。因此,四霉素可作为防治葡萄灰霉病的候选药剂,研究结果对两种杀菌剂的科学使用以及葡萄灰霉病的可持续防控具有重要的理论和实践意义。Gray mold,caused by Botrytis cinerea Pers.,is one of the most destructive grape diseases,which has influenced grape fruit yield and quality.To clarify sensitivity of B.cinerea to tetramycin and boscalid,we detected the half maximal effective concentration(EC)of tetramycin and boscalid against 60 isolates from Binchun,Wuhan and Beizhen by mycelial growth rate and spore germination methods,and analyzed the cross-resistance relationship between them.The results showed that baseline sensitivity of B.cinerea to tetramycin and boscalid were 0.245 and 1.115μg/mL,respectively.All the B.cinerea isolates exhibited sensitive to tetramycin,while 11.7%were resistant to boscalid.In addition,no cross resistance was found between tetramycin and boscalid(r=-0.040,P>0.05).Thus,tetramycin can be a candidate fungicide for gray mold control in field.The results are of significance in application of fungicides and sustainable control of gray mold.

关 键 词：葡萄灰霉病菌 四霉素 啶酰菌胺 敏感性 交互抗药性 

分 类 号：S436.631.1[农业科学—农业昆虫与害虫防治]