5-HT1A受体在翘嘴鳜中的表达及DNA甲基化分析  

Expression of 5-HT1A receptor and DNA methylation analysis in Chinese perch, Siniperca chuatsi

作　　者：庄武元 梁旭方[1,2] 肖倩倩 张志陆 蔡文静 ZHUANG Wuyuan;LIANG Xufang;XIAO Qianqian;ZHANG Zhilu;CAI Wenjing(College of Fisheries,Huazhong Agricultural University,Chinese Perch Research Center of Huazhong Agricultural University,Wuhan 430070,China;Engineering Research Center of Green development for Conventional Aquatic Biological Industry in the Yangtze River Economic Belt,Ministry of Education,Wuhan 430070,China)

机构地区：[1]华中农业大学水产学院,华中农业大学鳜鱼研究中心,湖北武汉430070 [2]长江经济带大宗水生生物产业绿色发展教育部工程研究中心,湖北武汉430070

出　　处：《中国水产科学》2022年第6期803-813,共11页Journal of Fishery Sciences of China

基　　金：国家自然科学基金面上项目(31772822);现代农业产业技术体系专项资金项目(CARS-46).

摘　　要：动物可通过学习记忆适应复杂的生存环境,而5-HT1A受体在学习记忆中发挥重要作用。为探究5-HT1A受体在翘嘴鳜(Siniperca chuatsi)驯化过程中摄食及食性巩固的作用,本研究采用同源序列比对的方式,在翘嘴鳜基因组获取了htr1a基因的序列,通过序列比对和进化树分析,发现其有两个亚型,分别命名为htr1aa和htr1ab,其编码的氨基酸序列与斑马鱼(Danio rerio)、青鳉(Oryzias latipes)具有较高的同源性,相似度都大于70%,进化关系上与狼鲈(Dicentrar chuslabrax)最为相近,表明翘嘴鳜htr1a基因在进化中具有较高的保守性。此外,本研究还分析了翘嘴鳜htr1a基因的表达和DNA甲基化。与经过一次驯化组相比,在二次驯化组中htr1aa基因表达显著降低(P<0.05),同时DNA甲基化也显著降低,而htr1ab基因在两组中表达没有显著变化(P>0.05)。而与摄食相关的食欲基因pomc的表达,二次驯化比一次驯化组显著降低(P<0.05)。以上结果说明,翘嘴鳜食性驯化过程中,可能通过htr1aa基因启动子区域的甲基化状态改变htr1aa的转录水平,从而影响学习记忆通路中关键因子抑制食欲因子pomc的表达。由此认为htr1aa的DNA甲基化可能在翘嘴鳜摄食相关基因表达上发挥重要调控作用。To explore the role of the 5-HT1A receptor in feeding and food consolidation during domestication of Chinese perch,Siniperca chuatsi,we obtained the htr1a gene sequence of the Chinese perch genome using homologous sequence alignment and evolutionary tree analysis.Htr1a has two isoforms,htr1aa and htr1ab,which encode amino acid sequences that have high homology with those of zebrafish(Danio rerio)and medaka(Oryzias latipes),with a similarity of more than 70%.The evolutionary relationship is closest between Chinese perch and wolf perch(Dicentrar chuslabrax).The results indicated that the htr1a gene of Chinese perch is highly conserved in evolution.The expression and methylation of the htr1a gene in mandarin fish were also analyzed.Compared with the first domestication group,the expression of the htr1aa gene and DNA methylation were significantly decreased in the second domestication group(P<0.05),while htr1ab gene expression was not significantly different between the two groups(P>0.05).The expression of pomc was related to food intake,which was obviously lower than that of a domesticated animal(P<0.05).The above results indicated that methylation of the promoter region of the htr1aa gene may change its transcription level during food domestication of Chinese perch,thus affecting the expression of key factors in the learning and memory pathways,inhibiting appetite factor pomc.Therefore,DNA methylation of htr1aa may play an important role in regulating the expression level of feeding related genes in Chinese perch.The results of this study further elucidate the mechanism of learning and memory in the transformation of feeding habits in fish and provide a theoretical solution to the difficult and unstable domestication of Chinese perch.

关 键 词：翘嘴鳜 5-HT1A受体 DNA甲基化 POMC NPY 

分 类 号：S917[农业科学—水产科学]