离子液体型表面活性剂的胶束电动色谱法手性拆分4种酸性药物  

Chiral separation of four acidic drugs by micellar electrokinetic chromatography with ionic liquid-based surfactant

作　　者：杨轲 于涛 YANG Ke;YU Tao(School of Science,China Pharmaceutical University,Nanjing 210098,China)

机构地区：[1]中国药科大学理学院,南京210098

出　　处：《中国药科大学学报》2022年第6期710-715,共6页Journal of China Pharmaceutical University

基　　金：国家自然科学基金资助项目(No.82073809);中国药科大学课程思政示范项目(仪器分析实验)资助

摘　　要：以葡萄糖基-β-环糊精(Glu-β-CD)作为手性选择剂,N-丁基-N-甲基吡咯烷十二烷基硫酸盐([C_(4)MP][C_(12)SO_(4)])离子液体表面活性剂形成胶束作为准固定相,在低pH条件下构建胶束电动毛细管色谱(MEKC)用于4种酸性药物萘普生、华法令、酮洛芬和布洛芬的手性拆分。在相同条件下,与基于传统表面活性剂十二烷基硫酸钠(SDS)的MEKC体系相比,[C_(4)MP][C_(12)SO_(4)]体系能大大提高药物的分离度,且使得一些原本不能拆分的药物得到部分分离。本研究考察了有机溶剂种类和比例、运行缓冲溶液浓度与pH、手性选择剂浓度、离子液体表面活性剂浓度、运行电压等影响手性拆分的因素,探索了离子液体表面活性剂在MEKC体系中的分离潜能,为手性药物的分离提供参考。In this paper, micellar electrokinetic chromatography(MEKC) with glucose-β-cyclodextrin(Glu-β-CD) as chiral selector and ionic liquid surfactant N-butyl-N-methyl pyrrolidine lauryl sulfate([C_(4)MP] [C_(12)SO_(4)]) micelles formed at low pH as a pseudo stationary phase was applied for the chiral separation of four acidic drugs naproxen,warfarin, ketoprofen and ibuprofen. Under the same conditions,significantly improved separation of tested drug enantiomers was achieved with the MEKC system based on [C_(4)MP][C_(12)SO_(4)] compared with the system based on the conventional surfactant sodium dodecyl sulfate(SDS). Several primary parameters affecting enantioseparation such as type and proportion of organic modifier, concentration and pH of the running buffer, concentration of chiral selector,concentration of ionic liquid surfactant and applied voltage were systematically investigated.

关 键 词：毛细管电泳 离子液体 胶束电动毛细管色谱 表面活性剂 手性拆分 酸性药物 

分 类 号：R917[医药卫生—药物分析学]