Single-molecular methodologies for the physical biology of protein machines  

作　　者：Shuang Wang Ying Lu Ming Li 王爽;陆颖;李明(Beijing National Laboratory for Condensed Matter Physics,Institute of Physics,Chinese Academy of Sciences,Beijing 100190,China)

机构地区：[1]Beijing National Laboratory for Condensed Matter Physics,Institute of Physics,Chinese Academy of Sciences,Beijing 100190,China

出　　处：《Chinese Physics B》2022年第12期693-701,共9页中国物理B（英文版）

基　　金：supported by the National Key Research and Development Program of China(Grant No.2019YFA0709304);the National Natural Science Foundation of China(Grant Nos.12090051 and 12022409);the Strategic Priority Research Program of the Chinese Academy of Sciences(Grant No.XDB37000000);the Youth Innovation Promotion Association of CAS(Grant Nos.2021009 and Y2021003)

摘　　要：Physical biology is an interdisciplinary field that bridges biology with physical sciences and engineering.Singlemolecule physical biology focuses on dynamics of individual biomolecules and complexes,aiming to answering basic questions about their functions and mechanisms.It takes advantages of physical methodologies to gain quantitative understanding of biological processes,often engaging precise physical measurements of reconstructed objects to avoid interference from unnecessary complications.In this review,we(i)briefly introduce concepts of single-molecule physical biology,(ii)describe extensively used single-molecule methodologies that have been developed to address key questions in two important objects of single-molecule physical biology,namely,nucleic acid-interacting proteins and membraneinteracting proteins,and(iii)show by a few successful examples how one may use single-molecule methods to deepen our understanding of protein machines.

关 键 词：physical biology single-molecules research object reconstruction precise measurements 

分 类 号：Q51[生物学—生物化学]