狮头鹅17β-HSD2基因SNPs位点的筛查及生物信息学分析  

SNPs Screening of 17β-HSD2 Gene and Bioinformatics Analysis in Shitou Goose

作　　者：陈新企 姜平 周光现 王慧芳 李立先 陈灿杰 贾汝敏[1] 赵志辉 CHEN Xinqi;JIANG Ping;ZHOU Guangxian;WANG Huifang;LI Lixian;CHEN Canjie;JIA Rumin;ZHAO Zhihui(College of Coastal Agricultural Sciences,Guangdong Ocean University,Zhanjiang,Guangdong 524088;Raoping Fubin Shitou Goose Breeding Base Corporation,Chaozhou,Guangdong 521000;Shantou Chenghai Chaopeng Ecological Breeding Co.,Ltd.,Shantou,Guangdong 515000)

机构地区：[1]广东海洋大学滨海农业学院,广东湛江524088 [2]饶平浮滨狮头鹅原种繁育基地有限公司,广东潮州521000 [3]汕头市澄海区潮鹏生态养殖有限公司,广东汕头515000

出　　处：《中国家禽》2022年第5期8-13,共6页China Poultry

基　　金：广东省现代农业产业技术体系创新团队(2019KJ137、2020KJ137);校企合作项目(B15337);汕头市科技局现代农业重点项目(210729156901723)

摘　　要：为筛选狮头鹅17β-羟基类固醇脱氢酶Ⅱ基因(17β-HSD2)的SNPs位点并进行生物信息学分析,采用直接测序法检测17β-HSD2基因外显子5区域的SNPs位点,并利用生物信息学软件分析突变前后mRNA二级结构的变化。结果表明,在狮头鹅群体中共发现6个SNPs,仅Exon5-351 G>A为错义突变。17β-HSD2基因突变前后mRNA最小自由能(MFE)、质心二级、含有假结的mRNA二级与折叠结构,均发生变化,且MFE自由能上升,结构稳定性变弱。结果可为后续探究17β-HSD2基因对狮头鹅繁殖性状的影响机制提供基础数据。In order to analyze the SNPs sites of 17β-Hydroxysteroid dehydrogenaseⅡ(17β-HSD2 gene)in Shitou goose,bioinformatics,SNPs in exon5 of 17β-HSD2 gene were detected by direct sequencing,and the changes of mRNA secondary structure before and after mutation were analyzed by bioinformatics software.The results showed that six SNPs were found in the Shitou goose population,and only exon5-351 G>A was a missense mutation.Before and after 17β-HSD2 gene mutation,the mRNA minimum free energy(MFE),centroid secondary,mRNA secondary and folding structure containing pseudoknots changed,and the MFE free energy increased and the structural stability weakened.The results could provide basic data for the follow-up study on the influence mechanism of 17β-HSD2 gene on reproductive traits of Shitou geese.

关 键 词：狮头鹅 17β-HSD2基因 SNPS 生物信息学 

分 类 号：S835.2[农业科学—畜牧学]