伊曲康唑对兔耳增生性瘢痕的影响  

Effects of Itraconazole on Hypertrophic Scars in Rabbit Ears

作　　者：高阳 袁嘉晨 南美兰 金哲虎[1,2] 金承龙 GAO Yang;YUAN Jiachen;NAN Meilan;JIN Zhehu;JIN Chenglong(Department of Dermatology,Yanbian University Hospital,Yanji 133000,China;Department of Medical Cosmetology,Yanbian University Hospital,Yanji 133000,China;Department of Dermatology,Suzhou Mylike Cosmetic Hospital,Suzhou 215000,China)

机构地区：[1]延边大学附属医院皮肤科,吉林延吉133000 [2]延边大学附属医院医学美容科,吉林延吉133000 [3]苏州美莱美容医院皮肤科,江苏苏州215000

出　　处：《中国皮肤性病学杂志》2022年第12期1356-1362,共7页The Chinese Journal of Dermatovenereology

基　　金：国家自然科学基金项目(81960561)

摘　　要：目的 探讨伊曲康唑(Itraconazole, ITZ)对兔耳增生性瘢痕的影响。方法 建立兔耳增生性瘢痕模型,25只新西兰大耳兔随机分为空白组(A组)、手术后即刻干预组(B1、B2组)、上皮化后干预组(C1、C2组),每小组5只兔,连续给药28 d。用药期间皮肤镜检查瘢痕血管消退情况;制取瘢痕标本,用苏木精-伊红染色法和Masson染色法观察病理学改变及测定瘢痕增生指数(HI)和胶原容积分数(CVF);Western blot和免疫组织化学法检测α-平滑肌肌动蛋白(α-SMA)、纤连蛋白(FN)、Ⅰ型胶原(ColⅠ)、Ⅲ型胶原(ColⅢ)、血管内皮生长因子(VEGF)的蛋白表达情况。结果 伊曲康唑干预后的分组瘢痕组织病理结构改善明显,HI和CVF降低,同时瘢痕组织中的α-SMA、FN、ColⅠ、ColⅢ及VEGF表达均显著下降。结论 伊曲康唑可以较好地抑制兔耳增生性瘢痕形成,探讨伊曲康唑作为增生性瘢痕的防治策略具有其可行性。Objective To investigate the effect of itraconazole(ITZ) on hypertrophic scar fibrosis in rabbit ears.Methods A rabbit ear hypertrophic scar model was established, and 25 New Zealand rabbits were randomly divided into blank group(group A), immediate intervention group after surgery(group B1, B2), and post-epithelialization intervention group(group C1, C2), there were 5 rabbits in each group, and the drugs were administered continuously for 28 days. During the treatment period, dermoscopy was used to examine the regression of scar blood vessels. The pathological changes were observed by hematoxylin-eosin staining(HE)and Masson staining, and the hypertrophic scar index(HI) and collagen volume fraction(CVF) were determined.The protein expressions of α-smooth muscle actin(α-SMA), fibronectin(FN), collagen Ⅰ(Col Ⅰ), collagen Ⅲ(Col Ⅲ), vascular endothelial growth factor(VEGF) were detected by Western blot and immunohistochemistry.Results The pathological structure of the scar tissue after itraconazole intervention was significantly improved, HI and CVF were decreased, and the expressions of α-SMA, FN, Col Ⅰ, Col Ⅲ and VEGF in the scar tissue were significantly decreased.Conclusion Itraconazole can inhibit the formation of hypertrophic scars in rabbit ears effectively, and it may be a prevention and treatment drug for hypertrophic scars.

关 键 词：增生性瘢痕 伊曲康唑 肌成纤维细胞 血管内皮生长因子 

分 类 号：R622[医药卫生—整形外科]