特应性皮炎差异表达基因的生物信息学分析  

Bioinformatic Analysis of Differentially Expressed Genes in Atopic Dermatitis

作　　者：汤柳 宋伟 李丹[1] 吴杰[1] TANG Liu;SONG Wei;LI Dan;WU Jie(Department of Pharmacy,Renmin Hospital of Wuhan University,Wuhan 430060,China)

机构地区：[1]武汉大学人民医院药学部,湖北武汉430060

出　　处：《中国皮肤性病学杂志》2022年第10期1118-1125,共8页The Chinese Journal of Dermatovenereology

基　　金：中央高校基本科研业务费专项资金资助(2042022kf1077);湖北省卫健委面上项目(WJ2021M147);国家自然科学基金青年基金(81803789)

摘　　要：目的运用生物信息学方法筛选特应性皮炎(atopic dermatitis)的差异表达基因(DEGs),明确其功能并探讨AD的发病机制。方法从GEO数据库中获取GSE120721、GSE16161、GSE6012与GSE329244基因芯片数据集,以“Adjust P<0.05,|logFC|>1”为条件筛选出各个数据集中AD皮损组织与正常人皮肤组织的差异表达基因(differentially expressed genes,DEGs),并通过合并去重获得共表达的DEGs。通过STRING 11.0在线工具以及Cytoscape 3.9.0软件构建共表达DEGs的蛋白互作(PPI)网络筛选关键基因,并对DEGs进行GO功能注释与KEGG通路富集分析。结果共筛选出75个共表达的DEGs;PPI网络分析获得GZMB、MMP12、ISG15、CCR7、CCNB1、MKI67、IRF7、BIRC3、CLEC7A、S100A9、CCL22、BIRC5、S100A7、SERPINB4等14个关键基因;GO富集结果表明共表达的DEGs主要参与炎症反应、防御反应、免疫反应等生物进程;KEGG通路分析筛选出显著富集的IL-17信号通路,且富集在该通路上的DEGs为S100A8、S100A7、S100A9、DEFB4A、FOSL1和MMP1,其中S100A7、S100A9属于枢纽基因。结论IL-17信号通路和枢纽基因S100A7、S100A9可能是AD发病机制中可进行干预的新靶点。Objective To identify differentially expressed genes(DEGs)and clarify their functions using bioinformatic analysis to explore the molecular mechanism of atopic dermatitis(AD).Methods GSE120721,GSE16161,GSE6012 and GSE329244 microarray datasets related to AD were downloaded from the Gene Expression Omnibus(GEO)database.Then,the differentially expressed genes(DEGs)between AD skin samples and healthy control samples were screened under the condition of"|logFC|>1 and adjusted P<0.05".At the same time,the DEGs shared by all four datasets were obtained by data merging and deduplication.Protein-protein interaction(PPI)network of shared DEGs was constructed using the STRING 11.0 database and Cytoscape 3.9.0 software.In addition,GO enrichment and KEGG pathways analyses of shared DEGs were performed.Results A total of 75 shared DEGs were filtered from the GEO database.PPI network analysis revealed 14 core genes,including GZMB,MMP12,ISG15,CCR7,CCNB1,MKI67,IRF7,BIRC3,CLEC7 A,S100 A9,CCL22,BIRC5,S100 A7,SERPINB4.The GO analysis showed that shared DEGs were mainly involved in biological processes,such as inflammatory response,defense response,immune response and others.The enrichment analysis of the KEGG pathway identified the IL-17 signaling pathway.DEGs enriched in this pathway included S100 A8,S100 A7,S100 A9,DEFB4 A,FOSL1 and MMP1,with S100 A7 and S100 A9 being two core genes.Conclusion The IL-17 signaling pathway and two core genes,S100 A7 and S100 A9,identified in this study may be new potential targets for therapeutic interventions for AD.

关 键 词：特应性皮炎 生物信息学 差异表达基因 富集分析 发病机制 

分 类 号：R758.2[医药卫生—皮肤病学与性病学]