Neutralization of five SARS-CoV-2 variants of concern by convalescent and BBIBP-CorV vaccinee serum  

作　　者：Yuqi Zhu Xinyi Yang Jingna Xun Jun Liu Qing Wen Yixiao Lin Xiaoting Shen Jun Chen Songhua Yuan Xiaying Zhao Jing Wang Hanyu Pan Jinlong Yang Zhiming Liang Yue Liang Qinru Lin Huitong Liang Min Li Jianping Liu Yinzhong Shen Xiaoyan Zhang Pengfei Wang Daru Lu Chunhua Yin Jianqing Xu Shibo Jiang Hongzhou Lu Huanzhang Zhu 

机构地区：[1]State Key Laboratory of Genetic Engineering and Engineering Research Center of Gene Technology,Ministry of Education,Institute of Genetics,School of Life Sciences,Fudan University,Shanghai,200438,China [2]Scientific Research Center,Shanghai Public Health Clinical Center,Fudan University,Shanghai,201508,China [3]Fubio(Suzhou)Biomedical Technology Co.,Ltd,Suzou,215300,China [4]Department of Infectious Diseases and Immunology,Shanghai Public Health Clinical Center,Fudan University,Shanghai,201508,China [5]Key Laboratory of Medical Molecular Virology(MOE/NHC/CAMS),School of Basic Medical Sciences,Fudan University,Shanghai,200032,China [6]Department of Infectious Diseases and Nursing Research Institution,National Clinical Research Center for Infectious Diseases,The Third People's Hospital of Shenzhen,Shenzhen,518112,China

出　　处：《Virologica Sinica》2022年第6期831-841,共11页中国病毒学（英文版）

基　　金：supported by the National Natural Science Foundation of China (82041001, 81761128020)

摘　　要：The prevalence of SARS-CoV-2 variants of concern(VOCs) is still escalating throughout the world. However, the level of neutralization of the inactivated viral vaccine recipients’ sera and convalescent sera against all VOCs,including B.1.1.7(Alpha), B.1.351(Beta), P.1(Gamma), B.1.617.2(Delta), and B.1.1.529(Omicron) remains to be lack of comparative analysis. Therefore, we constructed pseudoviruses of five VOCs using a lentiviral-based system and analyzed their viral infectivity and neutralization resistance to convalescent and BBIBP-CorV vaccinee serum at different times. Our results show that, compared with the wild-type strain(WT), five VOC pseudoviruses showed higher infection, of which B.1.617.2 and B.1.1.529 variant pseudoviruses exhibited higher infection rates than wild-type or other VOC strains, respectively. Sera from 10 vaccinated individuals at the 1, 3and 5-month post second dose or from 10 convalescent at 14 and 200 days after discharge retained neutralizing activity against all strains but exhibited decreased neutralization activity significantly against the five VOC variant pseudoviruses over time compared to WT. Notably, 100%(30/30) of the vaccinee serum samples showed more than a 2.5-fold reduction in neutralizing activity against B.1.1.529, and 90%(18/20) of the convalescent serum samples showed more than 2.5-fold reduction in neutralization against B.1.1.529. These findings demonstrate the reduced protection against the VOCs in vaccinated and convalescent individuals over time, indicating that it is necessary to have a booster shot and develop new vaccines capable of eliciting broad neutralization antibodies.

关 键 词：SARS-CoV-2 Variants of concern(VOCs) Convalescent BBIBP-CorV vaccine NEUTRALIZATION 

分 类 号：R186[医药卫生—流行病学]