Seroepidemiologic study on convalescent sera from dengue fever patients in Jinghong,Yunnan  

作　　者：Yingshuo Ma Man Li Lyu Xie Na Gao Dongying Fan Kaihao Feng Yao Yao Yong Zhou Ziyang Sheng Hongning Zhou Hui Chen Jing An 

机构地区：[1]Department of Microbiology,School of Basic Medical Sciences,Capital Medical University,Beijing,100069,China [2]Yunnan Institute of Parasitic Diseases,Pu’er,665000,China [3]People's Hospital of Xishuangbanna Dai Autonomous Prefecture,Xishuangbanna,666100,China [4]Xishuangbanna Mental Health Center,Xishuangbanna,666100,China [5]Yunnan Provincial Key Laboratory of Vector-Borne Diseases Control and Research and Yunnan Provincial Collaborative Innovation Center for Public Health and Disease Prevention and Control(YPCICPHDPC),Pu’er,665000,China [6]Experimental Center for Basic Medical Teaching,School of Basic Medical Sciences,Capital Medical University,Beijing,100069,China

出　　处：《Virologica Sinica》2022年第1期19-29,共11页中国病毒学（英文版）

基　　金：supported by the National Natural Science Foundation of China under grants 81772172,U1902210,81972979 and 81902048

摘　　要：After dengue virus(DENV)infection,antibody-dependent enhancement(ADE)is easy to occur when the neutralizing antibody(NAb)gradually decreases to a sub-neutralizing concentration.In this cohort surveillance,we utilized sera samples collected from dengue fever patients at different convalescent phases in Jinghong City,to investigate the dynamic change rule of DENV-specific antibodies,and to analyze the risk of ADE caused by secondary infection with heterologous serotypes DENVs.For baseline serosurvey,191 four-year and 99 six-year sera samples during convalescence were collected in 2017 and 2019,respectively.The positive rate of DENVspecific immunoglobulin G was 98.4%in 2017,which significantly decreased to 82.8%in 2019.The geometric mean titer(GMT)of NAb decreased from 1:155.35 to 1:46.66.Among 290 overall samples,73 paired consecutive samples were used for follow-up serosurvey.In four-year sera,the GMTs of NAb against DENV-3 and cross-reactive antibodies against DENV-1,DENV-2 and DENV-4 were 1:167.70,1:13.80,1:18.54 and 1:45.26,respectively,which decreased to 1:53.18,1:10.30,1:14.60 and 1:8.17 in six-year sera.In age-stratified analysis,due to the increasing number of ADE positive samples from 2017 to 2019 in 31–40 and 51–60 years groups,the risk of ADE in DENV-4 infection was positively associated with the extension of convalescent phase,and the odd ratio was higher than other groups.With the recovery period lengthened,the risk of secondary infection with DENV-1 and DENV-2 was reduced.Our results offer essential experimental data for risk prediction of severe dengue in hyper-endemic dengue areas,and provide crucial scientific insight for the development of effective dengue vaccines.

关 键 词：Dengue virus(DENV) SEROEPIDEMIOLOGY Neutralizing antibody(NAb) Cross-reactive antibody Antibody dependent enhancement(ADE) 

分 类 号：R512.8[医药卫生—内科学] R181.3[医药卫生—临床医学]