不同部位桂枝与肉桂对佐剂关节炎大鼠抗凝血作用的影响  

The effect of different directional branches of Cinnamomi Ramulus and Cinnamomi Cortex on anticoagulation activity in adjuvant arthritis rats

作　　者：韦露玲 杨凌霄 许立拔 任佳妮 黄玉妹 滕建北 Wei Luling;Yang Lingxiao;Xu Liba;Ren Jiani;Hua Yumei;Teng Jianbei(Hezhou Inspection And Testing Center,Hezhou542899;Guangxi Key Laboratory of Zhuang and Yao Ethnic Medicine,Nanning 530200,China;The people’s hospital of HeZhou,HeZhou 542800;Guangxi university of Chinese medicine,Nanning 530200;Collaborative Innovation Center of Zhuang and Yao Ethnic Medicine,Nanning 530200,China;Guangxi Zhuang Autonomous Region Ethnic Medicine Resources and Application Engineering Research Center,Nanning 530200,China)

机构地区：[1]贺州市检验检测中心,贺州542899 [2]广西壮瑶药重点实验室,广西南宁530200 [3]贺州市人民医院,贺州542800 [4]广西中医药大学,南宁530200 [5]壮瑶药协同创新中心,广西南宁530200 [6]广西壮族自治区民族药资源与应用工程研究中心,广西南宁530200

出　　处：《壮瑶药研究》2023年第2期224-228,390-391,共7页Research of Zhuang and Yao Ethnic Medicine

基　　金：广西中医药重点学科壮药学(GZXK-Z-20-64);广西重点研发计划项目(桂科AB21196016);广西科技基地和人才专项(桂科AD21238031)

摘　　要：目的:以不同部位桂枝和肉桂为研究对象,研究其对佐剂关节炎大鼠抗凝血作用,以期发现不同部位桂枝和肉桂药效的异同及为其活血化瘀作用机理提供前期基础。方法:参照《中国药典》(2020年版)对桂枝直径描述,设计按横切面直径选取不同取材直径的桂枝(A:0.3 cm以下;B:0.3~1 cm;C:1~4 cm);肉桂药材根据药典规定剥取、加工获得。采用水蒸气蒸馏法提取3组桂枝和肉桂中挥发性成分。除正常组外,其余大鼠注射弗氏完全佐剂(FCA)复制佐剂关节炎(AA)模型。5天后,选取80只造模成功大鼠随机分为8组,包括模型组,雷公藤多苷片组(20 mg/kg),桂枝挥发油A、B、C组(0.1 mL/kg)和肉桂挥发油3个剂量组(0.05,0.1和0.2 mL/kg),另取10只大鼠编入正常组。正常组和模型组给予橄榄油(5 mL/kg),其他组分别给予不同试药,1次/d,连续灌胃给药16 d,采用玻片法和玻璃毛细管法检测凝血时间。结果:不同部位桂枝和肉桂均可延长大鼠玻片法和玻璃毛细管法凝血时间,其中以桂枝B、C挥发油和肉桂挥发油0.1 mL/kg剂量组作用最显著。结论:不同部位桂枝和肉桂对佐剂关节炎大鼠都有抗凝血作用,桂枝B、C组和肉桂高剂量组具有明显的抗凝血作用。Objective:Three differently directional branches of Cinnamomi Ramulus and Cinnamomi Cortex were selected,and comparative study on their Anticoagulation on adjuvant arthritis in rats were used.In order to discover the differences and similarities in the pharmacological effects of Three differently directional branches of Cinnamomi Ramulus and Cinnamomi Cortex and provide a preliminary basis for their activating blood and resolving stasis.Methods:Differently directional branches of Cinnamomi Ramulus were selected according to the branche cross-sectional diameter(A:0.3 cm and below;B:0.3~1 cm;C:1~4 cm).According to the content determination method of cinnamaldehyde in Cinnamomi Cortex and Cinnamomi Ramulus in Chinese Pharmacopoeia(2020 Edition),The volatile oil components of Cinnamomi Ramulus A,B,C and Cinnamomi Cortex were extracted by steam distillation method.All the rats except the control group were treated by subcutaneously injecting freunds complete adjuvant(FCA)to establish adjuvant arthritis(AA)model.Five days later,eighty successful model rat were randomly divided into nine groups,including model group,Tripterygium wilfordii polyglycosides group(20 mg/kg),three Cinnamomi Ramulus volatile oil groups A,B and C(0.1mL/kg)and three Cinnamomi Cortex volatile oil groups(0.05,0.1 and 0.2 mL/kg),another ten rats without modeling were assigned into control group.Rats in control group and model group were received of olive oil(5 mL/kg),and rats in other groups were received different test drugs.all the rats were treated by gastric intubation for sixteen consecutive days,Glass slide method and glass capillary method were used to evaluate the clotting time in each rat.Results:Four volatile oil could obviously prolong the coagulation time by slide method and glass-tube method,Cinnamomi Ramulus B,C and Cinnamomi Cortex 100μg/mL group were the best.Conclusions:Three differently directional branches of Cinnamomi Ramulus and Cinnamomi Cortex have anticoagulant effects on adjuvant arthritis rats.Cinnamomi Ramulus B,C and Ci

关 键 词：桂枝 肉桂 佐剂关节炎 抗凝血作用 

分 类 号：R285.5[医药卫生—中药学]