HPLC法同时测定朝药肾炎草中4种黄酮碳苷成分的含量  

HPLC method for simultaneous determination of four flavonoid C-glycosides in Korean medicine Melandrium firmum Rohrb.

作　　者：丁舒研 朴梦花 于跃 郎明越 呼德日 李程程 全序胤 康东周[1] 郑明善[1] DING Shuyan;PIAO Menghua;YU Yue;LANG Mingyue;HU Deri;LI Chengcheng;QUAN Xuyin;KANG Dongzhou;ZHENG Mingshan(Yanbian University College of Pharmacy,Yanji 133002,Jilin,China)

机构地区：[1]延边大学药学院,吉林延吉133002

出　　处：《延边大学医学学报》2023年第3期173-176,共4页Journal of Medical Science Yanbian University

基　　金：国家自然科学基金地区项目(81660646);吉林省中医药管理局科技项目(2023088).

摘　　要：[目的]建立可同时测定朝药肾炎草中芹菜素-8-C-阿拉伯呋喃糖、异牡荆素、金圣草素-8-C-阿拉伯呋喃糖和异金雀花素4种黄酮碳苷成分含量的HPLC方法.[方法]采用SunFire C_(18)(5μm,4.6 mm×250 mm)色谱柱,流动相为0.1%(体积分数)冰乙酸的乙腈溶液(B)-0.1%(体积分数)乙酸水溶液(A),梯度洗脱,流动速度为1.0 mL/min,检测波长为335 nm,柱温为50℃,进样量为10μL.[结果]芹菜素-8-C-阿拉伯呋喃糖、异牡荆素、金圣草素-8-C-阿拉伯呋喃糖和异金雀花素分别在0.42~28.00、0.30~20.00、0.90~20.00、0.83~25.14μg/mL的质量浓度范围内与峰面积的线性关系良好,r^(2)>0.9995.精密度、稳定性、重复性试验结果的RSD<2.0%;平均加样回收率在94.7%~107.9%之间,RSD<2.0%.[结论]所建立的方法简便快捷,准确有效,可为朝药肾炎草中黄酮碳苷类成分含量的测定提供参考.OBJECTIVE T o establish a HPLC method for simultaneous determination of 4 kinds of flavonoid C-glycosides apigenin-8-C-arabinofuranose,isovitexin,chrysoeriol-8-C-arabinofuranose and isoscoparin in Korean medicine Melandrium firmum Rohrb.METHODS T he chromatographic column was SunFire C_(18)(5μm,4.6 mm×250 mm),the mobile phase was 0.1%(volume fraction)glacial acetic acid acetonitrile solution(B)-0.1%(volume fraction)acetic acid aqueous solution(A)with gradient elution and flow rate of 1.0 mL/min.The detection wavelength was set at 335 nm,the column temperature was 50℃,and the sample size was 10μL.RESULTS Aapigenin-8-C-arabinofuranose,isovitexin,chrysoeriol-8-C-arabinofuranose and isoscoparin had a good linear relationship with the peak area within the mass concentration range of 0.42-28.00,0.30-20.00,0.90-20.00 and 0.83-25.14μg/mL,respectively,r^(2)>0.9995.RSDs of precision,stability and repeatability tests were all less than 2.0%.The average recoveries ranged from 94.7%to 107.9%,RSD<2.0%.CONCLUSION This method is simple,rapid,accurate and effective,and can provide a reference for the determination of flavonoid C-glycosides in Korean medicine Melandrium firmum Rohrb.

关 键 词：肾炎草 黄酮碳苷 HPLC 含量测定 

分 类 号：R284[医药卫生—中药学]