HPLC波长切换法同时测定通幽润燥丸中9种活性成分  

Simultaneous Determination of Nine Active Components in Tongyou Runzao Pills by HPLC with Wavelength Switching Method

作　　者：吕晋 崔庆德 方慧祥 LYU Jin;CUI Qingde;FANG Huixiang(Qujing Food and Drug Inspection and Testing Center,Qujing 655000,China)

机构地区：[1]曲靖市食品药品检验检测中心,云南曲靖655000

出　　处：《中国现代应用药学》2022年第13期1716-1720,共5页Chinese Journal of Modern Applied Pharmacy

摘　　要：目的采用HPLC波长切换法同时测定通幽润燥丸中柚皮苷、新橙皮苷、黄芩苷、汉黄芩苷、黄芩素、汉黄芩素、和厚朴酚、厚朴酚和大黄酚9种活性成分的含量。方法采用依利特Hypersil OSD2 C18色谱柱(250 mm×4.6 mm,5μm);以乙腈(A)-0.1%磷酸溶液(B)为流动相进行梯度洗脱;流速为1.0 mL·min^(-1);柱温为30℃;检测波长为275 m(柚皮苷、新橙皮苷、黄芩苷、汉黄芩苷、黄芩素、汉黄芩素)和220 m(和厚朴酚、厚朴酚、大黄酚);进样量10μL。结果9种活性成分的分离度较好。柚皮苷、新橙皮苷、黄芩苷、汉黄芩苷、黄芩素、汉黄芩素、和厚朴酚、厚朴酚和大黄酚进样量分别在0.76~22.94,0.47~14.21,1.46~43.66,0.24~7.11,0.51~15.36,0.13~3.93,0.053~1.58,0.30~8.95,0.38~11.38 mg·mL^(-1)内呈良好线性关系,平均加样回收率(n=6)分别为99.27%,100.04%,98.90%,100.10%,99.67%,98.75%,99.88%,99.59%,99.92%,RSD分别为0.12%,0.15%,0.41%,0.18%,0.28%,0.27%,0.65%,0.46%,0.24%。结论通幽润燥丸中9种成分地含量测定方法准确,灵敏度高,分离效果好,能较好地评价该品种的质量,为该药物全面的质量控制提供了科学依据。OBJECTIVE To determine the content of naringin,neohesperidin,baicalin,wogonoside,baicalein,wogonin,honokiol,magnolol and chrysophanol in Tongyou Runzao pills by HPLC wavelength switching method.METHODS The column of illite Hypersil OSD2 C18(250 mm×4.6 mm,5μm)was used;the mobile phase of acetonitril(A)-0.1%phosphoric acid solution(B)was used for gradient elution;the flow rate was 1.0 mL·min^(-1);the column temperature was 30℃;the detection wavelength was 275 nm(naringin,neohesperidin,baicalin,wogonoside,baicalein,wogonin)and 220 nm(honokiol,magnolol,chrysophanol);the injection volume was 10μL.RESULTS The separation of the nine active constituents was good.The linear range was 0.76-22.94,0.47-14.21,1.46-43.66,0.24-7.11,0.51-15.36,0.13-3.93,0.053-1.58,0.30-8.95,0.38-11.38 mg·mL^(-1)for naringin,neohesperidin,baicalin,wogonoside,baicalein,wogonin,honokiol,magnolol and chrysophanol,respectively.The average recoveries(n=6)were 99.27%,100.04%,98.90%,100.10%,99.67%,98.75%,99.88%,99.59%,99.92%,respectively with RSDs of the recoveries were 0.12%,0.15%,0.41%,0.18%,0.28%,0.27%,0.65%,0.46%,0.24%,respectively.CONCLUSION The method for the determination of 9 components in Tongyou Runzao pills is accurate,sensitive and has good separation effect.It can better evaluate the quality of the drug and provide a scientific basis for the overall quality control of drug.

关 键 词：通幽润燥丸 高效液相色谱法 波长切换法 活性成分 含量测定 

分 类 号：R286.0[医药卫生—中药学]