当归挥发油通过抑制PI3K/Akt/mTOR信号转导通路影响人结肠癌LOVO细胞自噬研究  

Study on Volatile Oil of Angelicae Sinensis Radix Affects Autophagy in Human Colorectal Cancer LOVO Cells by Inhibiting PI3K/Akt/mTOR Signal Transduction Pathway

作　　者：朱丽娟 罗建云 宋润泽[4] 王丽娟 张安平[4] 臧凯宏 ZHU Lijuan;LUO Jianyun;SONG Runze;WANG Lijuan;ZHANG Anping;ZANG Kaihong(College of Pharmacy,Gansu University of Chinese Medicine,Lanzhou 730000,China;Gansu Province Key Laboratory of TCM Pharmacology and Toxicology,Lanzhou 730000,China;Xi’an Healthcare Security Bureau of Shaanxi Province,Xi’an 710021,China;Department of Vascular Surgery,Lanzhou University Second Hospital,Lanzhou 730000,China)

机构地区：[1]甘肃中医药大学药学院,兰州730000 [2]甘肃省中药药理与毒理学重点实验室,兰州730000 [3]陕西省西安市医疗保障局,西安710021 [4]兰州大学第二医院血管外科,兰州730000

出　　处：《中国现代应用药学》2022年第4期437-441,共5页Chinese Journal of Modern Applied Pharmacy

基　　金：甘肃省高等学校科研基金资助项目(2018-A050);甘肃省中药药理与毒理学重点实验室开放基金资助项目(ZDSYS-KJ-2018-008)

摘　　要：目的研究当归挥发油(volatile oil of Angelicae Sinensis Radix,VOAS)对人结直肠癌LOVO细胞自噬的影响及其分子机制。方法用不同浓度的VOAS(6.25,12.5,25,50和100μg·mL^(-1))作用于人结肠癌LOVO细胞48 h,CCK8比色法检测VOAS对LOVO细胞增殖的抑制作用。将VOAS 50μg·mL^(-1)作用于LOVO细胞,AO荧光染色和透射电镜观察细胞自噬情况;Western blotting观察VOAS对自噬相关蛋白LC3B、Beclin-1、Atg5及PI3K/Akt/mTOR信号转导通路的影响。结果6.25~100μg·mL^(-1)的VOAS能够抑制LOVO细胞的增殖,具有浓度依赖性;50μg·mL^(-1)的VOAS能够促进LOVO细胞的自噬,上调自噬相关蛋白LC3B-Ⅱ、Beclin-1及Atg5的表达,同时下调PI3K、p-Akt、p-mTOR蛋白的表达。结论VOAS能够促进人结肠癌LOVO细胞自噬,其机制可能与抑制PI3K/Akt/mTOR信号转导通路有关。OBJECTIVE To study the effect and mechanism of volatile oil of Angelicae Sinensis Radix(VOAS)on autophagy in human colorectal cancer LOVO cells.METHODS Human colorectal cancer LOVO cells were treated with various concentrations of VOAS(6.25,12.5,25,50 and 100μg·mL^(-1))for 48 h,CCK8 assay was used to detect the effect of VOAS on the proliferation inhibition effect of cells.When treated with VOAS(50μg·mL^(-1)),the effect of VOAS on autophagy was observed by acridine orange staining and electron microscopy,Western blotting was used to detect the expression of autophagy hallmark protein(LC3B,Beclin-1,Atg5)and PI3K/Akt/mTOR signal pathway.RESULTS The 6.25-100μg·mL^(-1)VOAS could significantly inhibit the proliferation of LOVO cells in dose-dependent manner,and VOAS(50μg·mL^(-1))could promote autophagy.Furthermore,VOAS(50μg·mL^(-1))could up-regulate the expression of LC3B-Ⅱ,Beclin-1 and Atg5 protein that related to autophagy,and down-regulate the expression of PI3K,p-Akt and p-mTOR protein.CONCLUSION VOAS may induce autophagy in human colorectal cancer LOVO cells through inhibiting PI3K/Akt/mTOR signal transduction pathway.

关 键 词：当归挥发油 LOVO细胞 自噬 PI3K/Akt/mTOR信号转导通路 

分 类 号：R285[医药卫生—中药学]