夏枯草通过调控MicroRNA-146b-3p对BRAF^(V600E)基因突变型甲状腺乳头状癌细胞生物学特性的影响  

Effects of Prunella vulgaris on Biological Characteristics of Thyroid Papillary Carcinoma Cells with BRAF^(V600E) Gene Mutation by Regulating MicroRNA-146b-3p

作　　者：栗粟 陈红跃[1] 武红园 杨萌萌 娄永庆 段祥凯 王心雨 LI Su;CHEN Hong-yue;WU Hong-yuan;YANG Meng-meng;LOU Yong-qing;DUAN Xiang-kai;WANG Xin-yu(Henan Province Hospital of Traditional Chinese Medicine,Zhengzhou Henan 450002,China;Second Clinical Medical College of Henan University of Traditional Chinese Medicine,Zhengzhou Henan 450002,China)

机构地区：[1]河南省中医院,河南郑州450002 [2]河南中医药大学第二临床医学院,河南郑州450002

出　　处：《时珍国医国药》2023年第5期1084-1088,共5页Lishizhen Medicine and Materia Medica Research

基　　金：河南省中医药科学研究专项课题(20-21ZY2051,20-21ZY2203)

摘　　要：目的探索夏枯草通过调控MicroRNA-146b-3p对BRAF^(V600E)基因突变型甲状腺乳头状癌细胞(B-CPAP细胞)增殖、侵袭及迁移生物学特性的影响。方法将夏枯草作用于B-CPAP细胞并设置对照组(转染miRNA抑制剂等),通过qRT-PCR技术检测夏枯草对B-CPAP细胞MicroRNA-146b-3p表达水平的影响;再分别采用CCK-8、Transwell及细胞划痕实验,检测夏枯草通过影响MicroRNA-146b-3p的表达水平对B-CPAP细胞增殖、侵袭以及迁移能力的影响。结果B-CPAP细胞microRNA-146b-3p的表达水平约为正常甲状腺细胞的4倍;1.0 mg/mL的夏枯草溶液使B-CPAP细胞microRNA-146b-3p表达水平降低约50%,通过降低microRNA-146b-3p的表达水平,使B-CPAP细胞的增殖、侵袭和迁移力分别降低约38%、80%和75%。结论夏枯草可通过下调MicroRNA-146b-3p的表达水平,抑制B-CPAP细胞的增殖,并降低其侵袭和迁移能力。Objective To explore the effects of Prunella vulgaris on the proliferation,invasion and migration of BRAF^(V600E) mutant thyroid papillary carcinoma cells(B-CPAP cells)by regulating microRNA-146b-3p.Methods Prunella vulgaris was applied to B-CPAP cells and the control group was set up(transfection of miRNA inhibitors,etc.).The effect of Prunella vulgaris on the expression of microRNA-146b-3p in B-CPAP cells was detected by qRT-PCR;Then CCK-8,Transwell and cell scratch tests were used to detect the effect of Prunella vulgaris on the proliferation,invasion and migration of B-CPAP cells by affecting the expression level of microRNA-146b-3p.Results The expression level of microRNA-146b-3p in B-CPAP cells was about 4 times that in normal thyroid cells;1.0 mg/mL Prunella vulgaris solution reduced the expression level of microRNA-146b-3p in B-CPAP cells by about 50%.By reducing the expression level of microRNA-146b-3p,the proliferation,invasion and migration of B-CPAP cells were reduced by about 38%,80%and 75%respectively.Conclusion Prunella vulgaris can inhibit the proliferation of B-CPAP cells and reduce their invasion and migration ability by down regulating the expression of microrna-146b-3p.

关 键 词：夏枯草 甲状腺乳头状癌 微小RNA-146b-3p 增殖 侵袭 迁移 

分 类 号：R285.5[医药卫生—中药学]