藤龙补中汤对大肠癌细胞全基因组启动子甲基化影响  

Effects of Teng-Long-Bu-Zhong-Tang on Global Promoter Methylation in Colorectal Carcinoma Cells

作　　者：胡兵[1] 安红梅[1] 闫霞[1] 郑佳露[1] 黄晓伟[1] 李淼 HU Bing;AN Hong-mei;YAN Xia;ZHENG Jia-lu;HUANG Xiao-wei;LI Miao(Longhua Hospital,Shanghai University of Traditional Chinese Medicine,Shanghai 200032,China)

机构地区：[1]上海中医药大学附属龙华医院,上海200032

出　　处：《时珍国医国药》2018年第11期2564-2566,共3页Lishizhen Medicine and Materia Medica Research

基　　金：国家自然科学基金(81273726,81473625);上海市科技支撑项目(16401902500)

摘　　要：目的观察藤龙补中汤对人大肠癌RKO细胞全基因组启动子甲基化的影响。方法采用藤龙补中汤处理大肠癌RKO细胞;试剂盒抽提DNA,免疫沉淀富集甲基化DNA,基因芯片检测全基因组基因启动子甲基化,Onto-Tools软件进行生物信息学分析。结果藤龙补中汤作用后,RKO细胞有6628个差异甲基化基因,其中甲基化基因3493个,去甲基化基因3135个。Ontology分析差异甲基化基因结果显示有4810个结构基因,4622个功能基因和4323个生物过程基因。在信号通路方面,有1103个差异甲基化基因,涉及信号通路75个,包括癌通路、MAPK通路、细胞因子-受体通路等信号通路。结论藤龙补中汤可调控大肠癌细胞多个基因启动子的甲基化,涉及多个生物过程与信号通路;基因启动子甲基化调控是藤龙补中汤作用的重要机制。Objective To observe the effects of Teng-Long-Bu-Zhong-Tang(TLBZT)on global promoter methylation in human colorectal carcinoma RKO cells.Methods RKO cells were treated with TLBZT.DNA was extracted by commercial kit.Methylated DNA was enriched with immunoprecipitation.Global promoter methylation was detected by microarray,and analyzed by Onto-Tools software.Results TLBZT treatment resulted in 6628 differentially methylated genes in RKO cells including 3493 methylated genes and 3135 demethylated genes.Ontology analysis revealed that 4810,4622 and 4323 differentially methylated genes belong to cellular components,molecular function and biological process gene respectively.In signal pathways,there were1103 genes differentially methylated and involvement of 75 signal pathways including pathways in cancer,MAPK signal pathway,cytokine-cytokine receptor interaction and other pathways.Conclusion TLBZT regulates multiple gene promoter methylation which involvement of multiple biological processes and signal pathways.Regulation of gene promoter methylation is an important effective mechanism of TLBZT.

关 键 词：大肠癌 藤龙补中汤 作用机制 DNA甲基化 信号通路 

分 类 号：R285.5[医药卫生—中药学]