一种乙醇复方消毒液消毒效果研究  

Antisepsis effect of a compound disinfectant of alcohol

作　　者：宋江南[1] 戴俊斌 陈贵秋[1] 夏昕[1] 尹进[1] 高琼[1] 张莹莹[1] 阳志文 SONG Jiangnan;DAI Junbin;CHEN Guiqiu;XIA Xin;YIN Jin;GAO Qiong;ZHANG Yingying;YANG Zhiwen(Hunan Provincial Center for Disease Control and Prevention,Changsha,Hunan 410005,China)

机构地区：[1]湖南省疾病预防控制中心,湖南长沙410005

出　　处：《实用预防医学》2023年第10期1159-1163,共5页Practical Preventive Medicine

基　　金：湖南省卫健委科研项目(202212065126)

摘　　要：目的了解一种乙醇复方消毒液杀灭细菌、白色念珠菌及病毒灭活效果,为选择合格有效的手消毒剂进行手卫生消毒提供科学依据。方法采用理化检测、载体定量杀菌试验、病毒灭活试验及手和皮肤消毒现场试验对一种乙醇复方消毒液进行消毒效果评价。结果该消毒液pH值为7.49,原液含乙醇为71.7%(v/v)、过氧化氢含量为0.42%,有效成分含量下降率与病毒灭活稳定性试验结果符合要求。原液作用0.5 min后,对金黄色葡萄球菌ATCC6538、大肠杆菌8099和铜绿假单胞菌ATCC15442的杀灭对数值均大于5.0,对白色念珠菌ATCC10231的杀灭对数值均大于4.0,作用0.5 min后,可有效灭活新型冠状病毒(SARS-CoV-2)和甲型H1N1(WSN)流感病毒,作用1.0 min后,有效灭活脊髓灰质炎病毒Ⅰ型(PV-1)疫苗株,禽流感病毒抵抗力较强,其灭活作用时间需延长至3 min。经30人次手和皮肤消毒现场试验,原液作用1.0 min对手和皮肤上的自然菌杀灭对数均值分别为2.55(1.36~2.91)和2.50(1.67~2.84)。结论该消毒液性能稳定,对细菌、白色念珠菌有快速杀灭效果,能够有效灭活甲型H1N1(WSN)流感病毒、新冠病毒、脊灰病毒和禽流感病毒,可广泛用于手卫生和皮肤消毒,降低感染发生。Objective To understand the efficacy of an ethanol compound disinfectant in killing bacteria,Candida albicans and viruses,and to provide a scientific basis for selecting qualified and effective hand disinfectants for hand hygiene disinfection.Methods Physical and chemical detection,carrier quantitative sterilization test,virus inactivation test and hand and skin disinfection field test were performed to assess the antisepsis effect of an ethanol compound disinfectant.Results The pH value of the disinfectant was 7.49,the original solution contained 71.7%(v/v)ethanol and 0.42%hydrogen peroxide.The decline rate of the active ingredient content met the requirements of the virus inactivation stability test.After the effect time of 0.5min,the logarithmic values of killing Staphylococcus aureus ATCC6538,Escherichia coli 8099 and Pseudomonas aeruginosa ATCC15442 were all greater than 5.0,and the logarithmic values of killing Candida albicans ATCC10231 were all greater than 4.0.After the effect time of 0.5min,severe acute respiratory syndrome coronavirus 2(SARS-CoV-2)and H1N1(WSN)influenza viruses could be effectively inactivated.After the effect time of 1.0min,the poliovirus vaccine strain(PV-1)could be effectively inactivated.Avian influenza virus had strong resistance,and its inactivation time needed to be extended to 3 minutes.After 30 field tests of hand and skin disinfection,the logarithmic mean values of killing natural bacteria on hands and skin for 1.0min were 2.55(1.36-2.91)and 2.50(1.67-2.84)respectively.Conclusion The disinfectant has stable performance,and can quickly kill bacteria and Candida albicans and effectively inactivate H1N1(WSN)influenza virus,SARS-CoV-2,poliovirus and avian influenza virus.It can be widely used for hand and skin disinfection,and reduce the incidence of infections.

关 键 词：乙醇复方消毒液 细菌 白色念珠菌 新型冠状病毒(SARS-CoV-2)等病毒 消毒效果 

分 类 号：R187[医药卫生—流行病学]