基于细胞代谢组学技术的知母皂苷元作用于肝癌细胞HepG2机制研究  

Study on the mechanism of sarsasapogenin on HepG2 cells based on cell metabolomics technology

作　　者：刘洋洋 王凯迪 夏丹旎 关峥嵘 于若楠 王淼[1] LIU Yangyang;WANG Kaidi;XIA Danni;GUANG Zhengrong;YU Ruonan;WANG Miao(College of Life Science and Biopharmaceuticals,Shenyang Pharmaceutical University,Shenyang 110016,China;College of Chinese Medicine,Shenyang Pharmaceutical University,Shenyang 110016,China)

机构地区：[1]沈阳药科大学生命科学与生物制药学院,辽宁沈阳110016 [2]沈阳药科大学中药学院,辽宁沈阳110016

出　　处：《沈阳药科大学学报》2023年第2期164-172,共9页Journal of Shenyang Pharmaceutical University

基　　金：大学生创新创业训练计划资助项目(X202010163189)

摘　　要：目的通过细胞代谢组学实验探究知母皂苷元作用于肝癌HepG2的抗肿瘤机制。方法采用液质联用和核磁技术研究知母皂苷元作用于肝癌细胞HepG2后的代谢机制,应用正交偏最小二乘判别分析方法筛选潜在生物标志物,应用MetaboAnalyst和KEGG PATHWAY数据库查找相关的代谢途径。结果共鉴定筛选出谷氨酸、丙氨酸、天冬氨酸、柠檬酸盐和2-酮戊二酸等51种生物标志物,寻找到丙氨酸、天冬氨酸和谷氨酸代谢通路;苯丙氨酸、酪氨酸和色氨酸的生物合成代谢通路;酮体的合成与降解的代谢途径等5条主要代谢通路。结论知母皂苷元作用于HepG2细胞后,可以引起肝癌HepG2细胞内代谢物较为广泛的变化。其中代谢标志物主要涉及氨基酸代谢和能量代谢,最终抑制了肿瘤细胞的增殖,诱导细胞凋亡。Objective To explore the anti-tumor mechanism of sarsasapogenin on HepG2 liver cancer through cell metabolomics experiments.Methods UPLC-MS spectrometry and NMR magnetic technology were used to study the metabolic mechanism of sarsasapogenin on HepG2 cells.Orthogonal partial least squares discriminant analysis method was used to screen potential biomarkers.MetaboAnalyst and KEGG PATHWAY database were used to find relevant metabolism way.Results A total of 51 biomarkers such as glutamate,alanine,aspartate,citrate and 2-ketoglutarate were identified,and 5 major metabolic pathways such as the metabolic pathways of alanine,aspartate and glutamate;the biosynthetic metabolic pathways of phenylalanine,tyrosine and tryptophan;the synthesis and degradation of ketones were found.Conclusion After sarsasapogenin acts on HepG2 cells,it can cause extensive changes in metabolites in HepG2 cells.Metabolism markers mainly involve amino acid metabolism and energy metabolism,which ultimately inhibit the proliferation of tumor cells and induce cell apoptosis.

关 键 词：知母皂苷元 细胞代谢组学 核磁 液质联用 肝癌 

分 类 号：R917[医药卫生—药物分析学]