GC法同时测定南葶苈子中4种脂肪酸的含量  

Simultaneous determination of four fatty acid component in descuraina semen by GC

作　　者：叶皓[1] 李姣姣 郑博 王淼[2] 赵旻[1] 赵春杰[1] YE Hao;LI Jiaojiao;ZHENG Bo;WANG Miao;ZHAO Min;ZHAO Chunjie(School of Pharmacy,Shenyang Pharmaceutical University,Shenyang 110016,China;School of Life Science and Biopharmaceutics,Shenyang Pharmaceutical University,Shenyan 110016,China)

机构地区：[1]沈阳药科大学药学院,辽宁沈阳110016 [2]沈阳药科大学生命科学与生物制药学院,辽宁沈阳110016

出　　处：《沈阳药科大学学报》2020年第3期246-251,共6页Journal of Shenyang Pharmaceutical University

基　　金：国家基础科学人才培养基金资助项目(J1103606).

摘　　要：目的建立气相色谱法同时测定南葶苈子中棕榈酸、亚麻酸、顺-11-二十烯酸、芥酸4种脂肪酸的含量。方法样品衍生化后,采用DB-5毛细管柱(30 m×0.32 mm,0.5μm),程序升温,气化室温度为280℃,FID检测器温度为290℃,载气为高纯氮气,流速为5 mL·min-1,进样量为1.0μL。结果棕榈酸、亚麻酸、顺-11-二十烯酸、芥酸质量浓度分别在0.256~1.28(r=0.9992)、1.583~7.915(r=0.9990)、0.532~2.66(r=0.9997)、0.364~1.82(r=0.9992)g·L-1内呈良好的线性关系,平均回收率分别为98.3%、97.8%、97.7%、97.9%(RSD≤2.0%,n=9)。结论首次建立了南葶苈子中4种主要脂肪酸的含量测定方法,可为南葶苈子的质量控制提供依据。Objective To establish a method for the determination of the content of palmitic acid,linolenic acid,cis-11-eicoenoic acid and erucic acid in the descuraina semen by GC.Methods The GC separation was achieved on a DB-5(30 m×0.25 mm,0.25μm)column with a temperature program at the rate of 5 mL·min-1after derivatization.Carrier gas is high purity nitrogen.Flame-ionization detector temperature was 290℃and injection port temperature was 280℃.The injection volume was 1.0μL.Results The linear range of palmitic acid,linolenic acid,11-eicoenoic acid and erucic acid was 0.256-1.28(r=0.9992),1.583-7.915(r=0.9990),0.532-2.66(r=0.9997),0.364-1.82(r=0.9992)g·L-1,respectively.The average recovery(RSD≤2.0%,n=9)was 98.3%,97.8%,97.7%and 97.9%,respectively.Conclusion The method is simple,accurate,reproducible,and could be used for quality control of descuraina semen oil.

关 键 词：南葶苈子 气相色谱法 含量测定 脂肪酸 

分 类 号：R284.1[医药卫生—中药学]