高效液相色谱法同时测定复方木鸡颗粒中金雀花碱、苦参碱、槐果碱和槲皮苷的含量  

Simultaneous determination of cytosine,matrine,sophocarpine and quercitrin in Fufang Muji granules by HPLC

作　　者：门磊 张梦莹 胡文忠 MEN Lei;ZHANG Mengying;HU Wenzhong(College of Life Sciences,Dalian University of Nationalities,Dalian 116600,China;Key Laboratory of Biotechnology and Resource Utilization,Ministry of Education,Dalian University of Nationalities,Dalian,116600,China)

机构地区：[1]大连民族大学生命科学学院,辽宁大连116600 [2]大连民族大学生物技术与资源利用教育部重点实验室,辽宁大连116600

出　　处：《沈阳药科大学学报》2020年第2期131-135,共5页Journal of Shenyang Pharmaceutical University

摘　　要：目的建立复方木鸡颗粒中4种成分(金雀花碱、苦参碱、槐果碱和槲皮苷)含量的高效液相色谱法测定方法。方法采用Waters Xselect T3 C18(250 mm×4.6 mm,5μm)色谱柱;流动相为磷酸盐缓冲液(pH 6.8)-三乙胺(体积比70∶0.1)(A)-乙腈(B)-甲醇(C)进行梯度洗脱;流速1.0 mL·min-1,柱温30℃,检测波长220 nm。结果 4种成分的分离度良好,且在各自浓度范围内呈现出良好的线性关系。金雀花碱、苦参碱、槐果碱和槲皮苷在进样质量浓度分别为2.0~32.0、9.0~144.0、5.0~80.0和4.0~64.0 mg·L-1内呈良好线性关系,相关系数r≥0.999 0,平均回收率在95.3%~103.2%,RSD为3.8%~4.8%(n=9)。含量测定结果显示,3种生物碱和槲皮苷在不同批次中的含量比较稳定。结论该法简便、准确,分离效果好,可用于复方木鸡颗粒的多成分同时测定,为复方木鸡颗粒的质量控制提供方法参考。Objective To establish an HPLC method to simultaneously determine the contents of sophorine,matrine,sophocarpine and quercitrin in Fufang Muji granules,in order to provide basis for studying its quality standards.Methods An HPLC method was developed.Waters Xselect T3 C18 column(4.6 mm×250 mm,5 μm) was used and eluted with mobile phase of acetonitrile-methanol-phosphate buffer(pH6.8) at the flow rate of 1.0 mL·min-1.The wavelength was set at 220 nm.The column temperature was maintained at 30 ℃.Results The good linear relationships between the concentration and peak area were in the range of 2.0-32.0 mg·L-1 for cytosine,5.0-80.0 mg·L-1 for matrine,9.0-144.0 mg·L-1 for matrine and 4.0-64.0 mg·L-1 for quercitrin(r≥0.999 0),respectively.The average recoveries(n=9) were in the range of 95.3%-103.2% and RSD were in the range of 3.8%~4.8%.Conclusion The method is simple,feasible and reproducible and can be used for the quality control of Fufang Muji Granules.

关 键 词：复方木鸡颗粒 金雀花碱 苦参碱 槐果碱 槲皮苷 含量测定 

分 类 号：R286.0[医药卫生—中药学] O657.72[医药卫生—中医学]