秦皮药材HPLC指纹图谱研究  

Study on HPLC fingerprint of Cortex Fraxini

作　　者：潘红[1] 叶皓[2] 曾博文[3] 王淼[3] 赵星[2] 赵春杰[2] PAN Hong;YE Hao;ZENG Bowen;WANG Miao;ZHAO Xing;ZHAO Chunjie(Hospital of Shandong Technology and Business University,Yantai 264005,China;School of Pharmacy,Shenyang Pharmaceutical University,Shenyang 110016,China;School of Life and Biopharmaceutics,Shenyang Pharmaceutical University,Shenyang 110016,China)

机构地区：[1]山东工商学院医院,山东烟台264005 [2]沈阳药科大学药学院,辽宁沈阳110016 [3]沈阳药科大学生命科学与生物制药学院,辽宁沈阳110016

出　　处：《沈阳药科大学学报》2019年第10期878-881,共4页Journal of Shenyang Pharmaceutical University

基　　金：国家基础科学人才培养基金资助项目(J1103606).

摘　　要：目的采用高效液相色谱法建立中药药材秦皮的指纹图谱,为秦皮药材科学的质量控制提供参考依据。方法采用Inertsil C18色谱柱(250 mm×4.6 mm,5μm),以体积分数0.1%甲酸水-甲醇溶液为流动相进行梯度洗脱,流速为0.8 mL·min^-1,检测波长为254 nm,柱温30℃;建立秦皮药材指纹图谱,运用中国药典委员会"中药色谱指纹图谱相似度评价系统软件"对10批秦皮药材进行相似度评价,并对其中四个色谱峰进行指认,对10批不同来源秦皮药材进行聚类分析。结果10批秦皮药材指纹图谱中标定了18个共有色谱峰,相似度值均在0.9以上。结论该法所建立的指纹图谱方法精密、重复性好、稳定性强,对提高秦皮药材的整体质量控制提供参考依据。Objective To establish the HPLC fingerprint of Cortex Fraxini,one of the Chinese herbal medicine,which provides a reference for quality control of Cortex Fraxini scientifically.Methods The separation was performed on a Inertsil C18 column(250 mm×4.6 mm,5μm)with water(containing 0.1%formic acid)-methanol as the mobile phase by gradient elution at the flow rate of 0.8 mL·min^-1.The detection wavelength was 254 nm and the column temperature was 30℃.Fingerprints of Cortex Fraxini were established and the similarity of 10 batches of Cortex Fraxini was evaluated by using"System for Similarity Evaluation of Chromatographic Fingerprints of Traditional Chinese Medicines"of the Chinese Pharmacopoeia Commission.Results The fingerprint chromatogram had 18 common peaks,the four of the peaks were identified and the similarities of 10 batches of Cortex Fraxini were all above 0.9.Conclusion The established method for HPLC fingerprint of Cortex Fraxini is precise,reproducible and stable,which provides a reference basis for improving the overall quality evaluation of Cortex Fraxini.

关 键 词：秦皮 HPLC 指纹图谱 

分 类 号：R284.1[医药卫生—中药学] O657.72[医药卫生—中医学]