荧光实时定量PCR法检测流感病毒核酸的质控结果分析  

Analysis of quality control results for fluorescence real time quantitative PCR detection of influenza virus nucleic acid

作　　者：宋广萍 SONG Guang-Ping(Suifenhe Disease Prevention and Control Center Laboratory,Mudanjiang 157399,China)

机构地区：[1]绥芬河市疾病预防控制中心检验科,牡丹江157399

出　　处：《实验室检测》2024年第7期101-103,共3页Laboratory Testing

摘　　要：目的分析浅析荧光实时定量PCR法检测流感病毒核酸的质控结果。方法在2021年5月至2022年11月期间,120例流感病毒感染样本进行研究。对这些样本的流感病毒RNA进行了核酸抽提,并利用PCR实时荧光定量技术进行检测以评估其效果。结果运用该技术检测到乙型流感病毒44例,甲1型40例,以及甲3型36例。所有检测结果均符合分子生物学操作的质控标准,合格率达到100.0%。结论实时核酸PCR技术在临床流感病毒检测中被广泛应用。PCR实时荧光定量检测法在流感病毒核酸检测中的表现精确且迅速,适宜作为应对突发公共卫生事件的首选检测手段。Objective To analyze the quality control results of detecting influenza virus nucleic acid by fluorescence real-time quantitative PCR.Methods From May 2021 to November 2022,120 samples of influenza virus infection were studied.The RNA of influenza virus in these samples was extracted by nucleic acid and detected by PCR real-time fluorescence quantitative technique to evaluate its effect.Results 44 cases of influenza B virus,40 cases of influenza A 1 virus and 36 cases of influenza A 3 virus were detected by this technique.All the test results meet the quality control standards of molecular biology operation,and the qualified rate reaches 100.0%.Conclusion Real-time nucleic acid PCR technology is widely used in clinical influenza virus detection.PCR real-time fluorescence quantitative detection method is accurate and rapid in the detection of influenza virus nucleic acid,which is suitable as the first choice to deal with public health emergencies..

关 键 词：荧光实时定量 PCR法检测 流感病毒核酸 质控结果 

分 类 号：R446.5[医药卫生—诊断学] O657.3[医药卫生—临床医学]