阿片肽与慢性单纯性苔藓瘙痒发生的相关性研究  

Correlation between opioid peptides and chronic lichen simplex pruritus

作　　者：朱江婷[1] 叶俊儒[1] 彭科[1] 沈跃莉[1] 陆茂[1] ZHU Jiang-ting;YE Jun-ru;PENG Ke(Department of Dermatology,the First Affiliated Hospital of Chengdu Medical College,Chengdu,610050,China)

机构地区：[1]成都医学院第一附属医院皮肤科,成都610050

出　　处：《实用皮肤病学杂志》2023年第3期146-149,共4页Journal of Practical Dermatology

基　　金：四川省卫生厅立项科研课题计划(130392)

摘　　要：目的初步探讨阿片肽对慢性单纯性苔藓瘙痒的生物学作用及相关研究。方法选择2020年4月—2021年3月于成都医学院第一附属医院皮肤科门诊治疗的慢性单纯性苔藓瘙痒患者分为2组,瘙痒组50例、无瘙痒组45例,另选取健康献血员的血浆(n=40)作为对照。采用放射免疫测定法测定标本的神经肽含量,用酶联免疫吸附试验(ELISA)测定β-内啡肽、亮啡肽和强啡肽的含量,受试者特征曲线(ROC)分析β-内啡肽、亮啡肽和强啡肽预测慢性单纯性苔藓瘙痒患者曲线下面积(AUC)。结果与正常对照组相比,无瘙痒组、瘙痒组患者血浆β-内啡肽、亮啡肽、强啡肽水平依次升高,差异具有统计学意义(P<0.05);与无瘙痒组相比,瘙痒组患者血浆β-内啡肽、亮啡肽、强啡肽水平升高,差异具有统计学意义(P<0.05)。与轻度瘙痒组相比,重度瘙痒组患者血浆β-内啡肽、亮啡肽、强啡肽水平较高,差异具有统计学意义(P<0.05)。β-内啡肽、亮啡肽和强啡肽联合检测对慢性单纯性苔藓瘙痒诊断价值优于单一检测,差异具有统计学差异(P<0.05)。结论联合检测β-内啡肽、亮啡肽、强啡肽水平对于慢性单纯性苔藓瘙痒患者有较高的诊断价值,为后续探究阿片肽对慢性单纯性苔藓瘙痒病情发展的影响机制提供了数据支撑。Objective To preliminarily explore the biological effects of opioid peptides on chronic lichen simplex and related research.Methods A total of 135 patients with lichen simplex pruritus who were treated with the outpatient department of our hospital from April 2020 to March 2021 were selected.All patients with lichen simplex pruritus included in the study were divided into 2 groups according to the diagnosis of the patients,the pruritus group(n=50)and the no pruritus group(n=45),and the plasma of healthy blood donors(n=40)was selected as Control group;the neuropeptide content of the specimen was determined by radioimmunoassay,the content of β-endorphin,enkephalin and dynorphin was determined by ELISA method,and the receiver operating characteristic curve(ROC)was used to analyze β-endorphin,leuporphin and dynorphin predict the area under the curve(AUC)in patients with chronic lichen simplex pruritus.Results Compared with the normal group,the plasma levels of β-endorphin,leuporphin and dynorphin in the non-pruritic group and pruritus group were increased sequentially,with statistical significance(P<0.05).The plasma levels of β-endorphin,leuporphin and dynorphin in the pruritus group increased with statistical significance(P<0.05).Compared with the mild itching group,the plasma β-endorphin,leuporphin and dynorphin levels in the severe itching group were significantly higher(P<0.05).The combined detection of β-endorphin,leuporphin and dynorphin in the diagnosis of chronic lichen simplex pruritus was better than single detection,with statistical difference(P<0.05).Conclusion Combined monitoring of β-endorphin,leuporphin and dynorphin levels has high diagnostic value in patients with chronic lichen simplex pruritus,and provides data support for the subsequent exploration of the influence mechanism of opioid peptides on the development of chronic lichen simplex pruritus.

关 键 词：苔藓 单纯性 慢性 瘙痒 阿片肽 Β-内啡肽 亮啡肽 强啡肽 

分 类 号：R758.32[医药卫生—皮肤病学与性病学]