负载光敏剂的生物可降解纳米药物载体用于肿瘤的光动力治疗  

Application of Biodegradable Nano-drug Carriers Loaded With Photosensitizers in the Photodynamic Therapy of Tumors

作　　者：何艳梅 施文强 蔡胜胜 梅蘅 曹俊[1] HE Yan-mei;SHI Wen-piang;CAI Sheng-sheng;MEI Heng;CAO Jun(Biomaterials Engineering Research Center,Sichuan University,Chengdu Sichuan 610065,China)

机构地区：[1]四川大学生物材料工程研究中心,四川成都610065

出　　处：《当代化工》2020年第7期1351-1355,共5页Contemporary Chemical Industry

摘　　要：探讨了生物可降解纳米药物载体负载光敏剂的光动抗癌疗效。以左旋乳酸为单体,1,6-己二醇为引収剂,采用开环聚合方法合成左旋聚乳酸,再将带正电荷的三苯基膦通过酯化反应键合到聚乳酸末端,核磁氢谱结果表明成功地合成了三苯基膦改性的聚乳酸。该聚合物在水中可自组装形成表面带正电荷的纳米粒子(TppT),光敏剂二氢卟吩e6(Ce6)可通过π-π共轭、疏水等作用负载于TppT中形成表面为负电的纳米粒子(TppT@Ce6)。动态光散射和透射电镜结果表明,TppT@Ce6具有球形结构,且负载Ce6后粒径增加、电位降低。TppT@Ce6在10%的血清中的粒径和分布变化较小,具有良好的稳定性。细胞实验结果表明,TppT@Ce6在无激光照射时细胞生长率大于80%;激光照射后,表现出较强的细胞毒性。以上结果表明,本研究所构建的生物可降解纳米药物载体可实现高效的抗肿瘤效果。The photodynamic anti-cancer efficacy of biodegradable nanoparticles drug carrier loaded with photosensitizer was explored.With sinistral lactic acid as monomer,1,6-diol as initiator,levorotatory polylactic acid was compounded by ring opening polymerization method,then positively charged triphenylphosphine was bonded at the terminal of polylactic acid through esterification reaction.Hydrogen nuclear magnetic spectra results showed that the triphenylphosphine modified polylactic acid was successfully synthesized.The polymer could be self-assembled in water to form positively charged nanoparticles(TppT),and the photosensitizer chlorin e6(Ce6)could be loaded into the TppT byπ-πconjugation and hydrophobicity to form negatively charged nanoparticles(TppT@ce6).The results of dynamic light scattering and transmission electron microscopy showed that TppT@ce6 had spherical structure,the particle size increased and the potential decreased after the load of Ce6.The particle size and distribution of TppT@ce6 in 10%serum had little change and good stability.The survival rate of TppT@ce6 without laser irradiation was more than 80%.After laser irradiation,cells showed strong cytotoxicity.The above results indicated that the biodegradable nano-drug carrier constructed in this study achieved effective anti-tumor effect.

关 键 词：共轭 聚乳酸 光敏剂 光动力治疗 纳米药物载体 

分 类 号：TB383.1[一般工业技术—材料科学与工程] TQ460.4[化学工程—制药化工]