中药减肥降脂复方中黄酮提取方法的研究及含量测定  

Study on Determination of Flavonoids in Chinese Medicine Weight Loss and Lipid-lowering Compound

作　　者：王娅玲 陶然 徐凯旋 李佳怿 王悦 宋兴爽 王婕[1] 高世杰[1] WANG Ya-ling;TAO Ran;XU Kai-xuan;LI Jia-yi;WANG Yue;SONG Xing-shuang;WANG Jie;GAO Shi-jie(School of Pharmacy,Shandong University of Traditional Chinese Medicine,Jinan Shandong 250014,China)

机构地区：[1]山东中医药大学,山东济南250014

出　　处：《当代化工》2020年第6期1114-1117,共4页Contemporary Chemical Industry

基　　金：山东中医药大学大学生研究训练(SRT)计划资助项目(项目编号:2018030)

摘　　要：按照药典含量检测的相关规定,检测中药减肥降脂方中黄酮的含量并考察比色法的可靠性。采用了超声辅助乙醇回流法测定减肥降脂复方中黄酮的含量,所测曲线相关性良好,符合Lambert-Beer定律。减肥降脂复方中总黄酮质量分数为45.065 3 mg·g-1,精密度的相对标准偏差为1.80%,重复性的相对标准偏差为1.50%,稳定性的相对标准偏差为0.44%,平均加标回收率的相对标准偏差为102.20%,表明比色法检测减肥降脂复方中的黄酮含量符合相关规定。According to the relevant regulations of pharmacopoeia content detection,the content of flavonoids in weight-reducing and lipid-lowering prescriptions of traditional Chinese medicine was detected and the reliability of colorimetry was investigated.Ultrasonic-assisted ethanol reflux method was used to determine the content of flavonoids in weight-reducing and lipid-lowering compound.The measured curves have good correlation and conform to Lambert-Beer law.The content of total flavonoids in weight-reducing and lipid-lowering compound is 45.0653 mg·g-1,the relative standard deviation of precision is 1.80%,the relative standard deviation of repeatability is 1.50%,the relative standard deviation of stability is 0.44%,and the relative standard deviation of average standard addition recovery rate is 102.20%,which indicates that the content of flavonoids in weight-reducing and lipid-lowering compound detected by colorimetry conforms to relevant regulations.

关 键 词：中药纤体 总黄酮 减肥降脂 含量测定 方法学研究 

分 类 号：TQ464[化学工程—制药化工]