栓菌锰过氧化物酶对合成染料的生物脱色研究  

Biological Decolorization of Synthetic Dyes by Manganese Peroxidase from White Rot Fungus

作　　者：聂志强 席镜慧 杨秀清 NIE Zhiqiang;XI Jinghui;YANG Xiuqing(Key Laboratory of Chemical Biology and Molecular Engineering of the Ministry of Education,Institute of Biotechnology,Shanxi University,Taiyuan 030006,China)

机构地区：[1]山西大学生物技术研究所,化学生物学与分子工程教育部重点实验室,山西太原030006

出　　处：《山西大学学报（自然科学版）》2021年第4期798-805,共8页Journal of Shanxi University(Natural Science Edition)

基　　金：山西省应用基础研究计划(青年科技研究基金)资助项目(201901D211130);山西省高等学校科技创新项目(2017105)

摘　　要：采用单因素实验和响应面法对栓菌锰过氧化物酶(manganese peroxidase,MnP)脱色偶氮染料的条件进行了优化。结果表明,影响MnP生物脱色的因素排序为:酶浓度>染料浓度>温度>pH值。通过Box-Benhnken设计优化后最优生物脱色条件为:温度49℃,pH值4.6,染料浓度85.5 mg/L,酶浓度430 U/L,脱色率为84.5%。在此基础上,研究了MnP对染有天青A的棉织布的漂白脱色应用,结果发现MnP作用棉织布2 h脱色率为16%。这表明该MnP在偶氮和噻嗪染料废水生物脱色领域具有潜在的应用价值。The biological decolorization conditions of azo dyes by manganese peroxidase(MnP)from white rot fungus were optimized by single factor experiments and response surface method.The results showed that the importance of factors affecting biological decolorization was as follows:enzyme concentration>dye concentration>temperature>pH value.The optimal biological decolorization conditions were optimized by Box-Benhnken design:the temperature was at 49℃,the pH value was 4.6,the dye concentration was 85.5 mg/L,the enzyme concentration was 430 U/L,and the decolorization rate was 84.5%.Moreover,the application of bleaching and decolorization of cotton fabric dyed with azure A by MnP was studied.The results showed that the decolorization rate of cotton fabric treated with MnP for 2 h was 16%,it suggests that the MnP has potential application value in the field of biological decolorization of azo and thiazine dyes wastewater.

关 键 词：锰过氧化物酶 偶氮染料 噻嗪染料 生物脱色 漂白 

分 类 号：X703[环境科学与工程—环境工程]