Subchronic Oral Toxicity Evaluation of Sodium Dehydroacetate: A 90-day Repeated Dose Study in Rats  

作　　者：FANG Jin LIU Hai Bo ZHI Yuan FENG Yong Quan WANG Hui Ling CUI Wen Ming ZHANG Ji Yue WANG Hua Li YU Zhou JIA Xu Dong 

机构地区：[1]NHC Key Laboratory of Food Safety Risk Assessment,Chinese Academy of Medical Science Research Unit(2019RU014),China National Center for Food Safety Risk Assessment,Beijing 100021,China

出　　处：《Biomedical and Environmental Sciences》2022年第4期296-311,共16页生物医学与环境科学（英文版）

基　　金：supported by the National Key R&D Program of China[2019YFC1605203];China Food Safety Talent Competency Development Initiative:CFSA 523 Program

摘　　要：Objective The present study was undertaken to evaluate the subchronic oral toxicity of sodium dehydroacetate(DHA-Na)and to determine the point of departure(POD),which is a critical factor in the establishment of an acceptable dietary intake.Methods DHA-Na was administered once daily by gavage to Sprague–Dawley rats at dose levels of 0.0,31.0,62.0,and 124.0 mg/kg BW per day for 90 days,followed by a recovery period of 4 weeks in the control and 124.0 mg/kg BW per day groups.The outcome parameters were mortality,clinical observations,body weights,food consumption,hematology and clinical biochemistry,endocrine hormone levels,and ophthalmic,urinary,and histopathologic indicators.The benchmark dose(BMD)approach was applied to estimate the POD.Results Significant decreases were found in the 62.0 and 124.0 mg/kg BW groups in terms of the body weight and food utilization rate,whereas a significant increase was found in the thyroid stimulating hormone levels of the 124.0 mg/kg BW group.Importantly,the 95%lower confidence limit on the BMD of 51.7 mg/kg BW was modeled for a reduction in body weight.Conclusion The repeated-dose study indicated the slight systemic toxicity of DHA-Na at certain levels(62.0 and 124.0 mg/kg BW)after a 90-day oral exposure.

关 键 词：Sodium dehydroacetate Sub-chronic toxicity 90-day repeated oral dose test Benchmark dose Point of departure 

分 类 号：R994[医药卫生—毒理学]