京海黄鸡柔嫩艾美耳球虫感染后盲肠转录组分析  

RNA Sequencing Analysis of Cecum Tissues of Jinghai Yellow Chickens Infected by E. tenella

作　　者：于海亮 邹文斌 王晓慧 林雨鑫[1,2] 戴国俊[1] 张涛[1] 张跟喜[1] 谢恺舟[1] 王金玉[1] 施会强[3] YU Hai-liang;ZOU Wen-bin;WANG Xiao-hui;LIN Yu-xin;DAI Guo-jun;ZHANG Tao;ZHANG Gen-xi;XIE Kai-zhou;WANG Jin-yu;SHI Hui-qiang(College of Animal Science and Technology,Yangzhou University,Yangzhou 225009;Animal Husbandry and Veterinary Station of Kunshan City,Kunshan 215300;Jiangsu Jinghai Poultry Group Co.Ltd,Haimen 226100)

机构地区：[1]扬州大学动物科学与技术学院,扬州225009 [2]昆山市畜牧兽医站,昆山215300 [3]江苏京海禽业集团有限公司,海门226100

出　　处：《生物技术通报》2019年第11期64-71,共8页Biotechnology Bulletin

基　　金：江苏省农业三新工程(SXGC[2017]298);江苏现代农业产业技术体系建设专项资金(JATS[2018]303);“十二五”国家科技支撑计划(2014BAD13B02);江苏省高校优势学科建设工程(PAPD);国家现代农业产业技术体系专项资金(CARS-41-G23).

摘　　要：为了探究京海黄鸡感染柔嫩艾美尔球虫(E.tenella)后盲肠组织差异表达基因,以及球虫感染分子应答过程和免疫应答机制,试验采用RNA-seq技术对E.tenella感染和非感染组第7天的盲肠组织进行转录组测序,筛选差异表达基因,并进行差异基因的功能、通路富集分析。结果表明,在感染和非感染组中有显著差异的表达基因2830个(P<0.05),其中1419个基因上调,1411个基因下调。随机选取10个差异基因进行qRT-PCR验证,结果显示差异基因的表达倍数与RNA-seq检测结果显著相关(r=0.988,P<0.000),决定系数达0.975。GO分析表明,有2356个差异基因获得GO功能注释,显著富集的前30个GO terms主要涉及细胞交流、信号转导、血管生成、氧化还原酶活性等。KEGG分析发现差异基因显著富集的信号通路有黏着斑、细胞外基质-受体相互作用、过氧化物酶体增殖物激活受体等。这些通路中的差异基因有ANGPTL4、ACSL5、VEGFC、CD44和MAKP10等,提示这些基因在宿主柔嫩艾美耳球虫感染过程中发挥重要作用。This work aims to investigate the differentially expressed genes(DEGs)of cecal tissue of Jinghai yellow chickens infected with E.tenella and the molecular response process and immune response mechanism of the infection.The RNA-seq technique was used to sequence the cecal tissue of E.tenella-infected group and non-infected group on the 7 th day of post-infection,and the DEGs were screened for functional and pathway enrichment analysis.The results indicated that there were 2830 DEGs(P<0.05)between the infected and uninfected groups,of which 1419 were up-regulated and 1411 were down-regulated.Ten DEGs were randomly selected for qRT-PCR verification,and the results showed that the fold change of DEGs was highly consistent with the RNA-seq results(r=0.988,P<0.000),and the coefficient of determination reached 0.975.GO analysis showed that 2356 DEGs were functionally annotated,and the top 30 significantly enriched GO terms involved mainly cell communication,signal transduction,angiogenesis,and oxidoreductase activity.The KEGG analysis revealed that top significantly enriched signaling pathways included focal adhesions,extracellular matrix-receptor interactions and peroxisome proliferatoractivated receptors.Key DEGs in these pathways include ANGPTL4,ACSL5,VEGFC,CD44,and MAKP10,etc.,suggesting that these genes would play an important role in the infection of E.tenella.

关 键 词：京海黄鸡 柔嫩艾美尔球虫 盲肠 转录组测序 差异表达基因 

分 类 号：S858.31[农业科学—临床兽医学]