2种方法在血清β2-微球蛋白检测中的效果对比  

Comparison of two methods for detection of serum β2-microglobulin

作　　者：张拔山[1] 李飞 张锡波 莫海兴 丁爱娇 ZHANG Ba-shan;LI Fei;ZHANG Xi-bo;MO Hai-xing;DING Ai-jiao(Department of laboratory,Dongguan People's Hospital,Dongguan,Guangdong,523059,China)

机构地区：[1]东莞市人民医院检验科,广东东莞523059

出　　处：《现代生物医学进展》2019年第23期4524-4527,4596,共5页Progress in Modern Biomedicine

基　　金：吴阶平医学基金会临床科研专项资助基金(320.6750.17355).

摘　　要：目的:探讨散射免疫法和荧光免疫层析法在血清β2-微球蛋白中的检测效果对比观察及精确度。方法:选取2016年10月-2017年8月我院收治的肝肾功能损伤患者88例,对患者分别应用散射免疫法以及荧光免疫层析法进行检测。对比两组方法血清β2-微球蛋白的检测结果、灵敏度、特异性以及精确度。结果:检测结果方面,荧光免疫分析法患者的检测率明显高于散射免疫法,漏检率明显低于散射免疫法(P<0.05);荧光免疫层析法的灵敏度、特异性以及诊断符合率明显高于散射免疫法(P<0.05);各项指标水平方面,患者血清β2-微球蛋白、肌酐、尿酸以及尿素等指标的水平明显高于正常水平(P<0.05);精确度方面,荧光免疫层析法批间、批内的精确度明显高于散射免疫法(P<0.05)。结论:对患者应用荧光免疫层析法检测血清β2-微球蛋白,有较高的检测符合率、灵敏度以及准确度,能够满足临床检测过程中的要求,进而有利于患者的诊断和治疗,临床上应当进一步推广应用。Objective:To explore the detection effect and accuracy of scattering immunoassay and fluorescence immunochromatography for detection of serumβ2-microglobulin.Methods:88 patients with liver and kidney function injury admitted to our hospital from October 2016 to August 2017 were selected in this study.And the level of serumβ2-microglobulin was detected by scattering immunoassay and fluorescence immunochromatography,respectively.The detection results,sensitivity,specificity and accuracy of serumβ2-microglobulin were compared between the two methods.Results:The detection rate by fluorescence immunochromatography was significantly higher than that of scattering immunoassay,while the missed detection rate was significantly lower than that of the scattering immune method(P<0.05).The sensitivity,specificity and diagnostic coincidence rate of fluorescence immunotomography were significantly higher than those of scattering immunotomography(P<0.05).The levels of serumβ2-microglobulin,creatinine,uric acid and urea of these patients were significantly higher than normal(P<0.05).In terms of accuracy,the accuracy of fluorescence immunochromatography between and within batches was significantly higher than that of scattering immunoassay(P<0.05).Conclusion:The application of fluorescence immunochromatography for detection of serumβ2-microglobulin in patients has a higher detection coincidence rate,sensitivity and accuracy,which can meet the requirements of clinical detection process,and is beneficial to the diagnosis and treatment of patients.Therefore,fluorescence immunochromatography should be further popularized in clinical application.

关 键 词：散射免疫法 荧光免疫层析法 血清Β2-微球蛋白 检测效果 精确度 

分 类 号：R446.6[医药卫生—诊断学]