基于斑马鱼模型的补血生髓方对肿瘤相关性贫血和免疫功能低下的保护作用  

Protective Effect of Buxue Shengsui Recipe on Cancer Related Anemia and Immunohypofunction Based on the Zebrafish Model

作　　者：张硕 姜晓燕[1] 符映均 倪红辉[1] 杨水英[1] 吴育[1] 彭敏[1] ZHANG Shuo;JIANG Xiaoyan;FU Yingjun;NI Honghui;YANG Shuiying;WU Yu;PENG Min(Nantong Hospital of Traditional Chinese Medicine,Nantong 226001,China)

机构地区：[1]南通市中医院,南通226001

出　　处：《世界科学技术-中医药现代化》2024年第5期1320-1327,共8页Modernization of Traditional Chinese Medicine and Materia Medica-World Science and Technology

基　　金：江苏省卫生健康委员会科研项目(Z2021081):补血生髓方增强肿瘤相关性贫血小鼠的免疫,改善造血功能的机制研究,负责人:张硕

摘　　要：目的探讨补血生髓方对斑马鱼贫血和免疫损伤的影响及可能机制。方法以Tg(gata1a:DsRed)斑马鱼为实验对象,建立苯肼诱导的斑马鱼贫血模型,观察不同浓度补血生髓方对斑马鱼背部血管平均强度的影响。以Tg(lysc:DsRed)斑马鱼为实验对象,建立氯霉素、长春瑞滨诱导的斑马鱼免疫损伤模型,观察补血生髓方对斑马鱼尾部中性粒细胞数量的影响。采用Real time-PCR检测免疫相关细胞因子白细胞介素6(IL-6)、白细胞介素10(IL-10)、肿瘤坏死因子α(TNF-α)mRNA的表达。结果与模型组相比,0.5 mg生药量·mL^(-1)和2 mg生药量·mL^(-1)补血生髓方显著增加斑马鱼背部血管平均强度(P<0.01),且呈剂量依赖性。2 mg生药量·mL^(-1)补血生髓方显著逆转氯霉素、长春瑞滨造成的中性粒细胞减少(P<0.01)。与模型组相比,补血生髓方组斑马鱼IL-6、IL-10 mRNA表达显著增加(P<0.05或P<0.01),TNF-αmRNA表达显著降低(P<0.05)。结论补血生髓方能够改善苯肼诱导的斑马鱼贫血状态,且对氯霉素、长春瑞滨诱导的斑马鱼免疫损伤均具有明显的保护作用,与上调细胞因子IL-6、IL-10,下调TNF-α的表达有关。Objective To explore the effects of Buxue Shengsui Recipe on anemia and immune injury in zebrafish and its mechanism.Methods Tg(gata1a:DsRed)zebrafish was used as experimental subjects to establish phenylhydrazineinduced zebrafish anemia model.To observe the effect of different concentrations of Buxue Shengsui Recipe on the average strength of zebrafish dorsal blood vessels.Besides,Tg(lysc:DsRed)zebrafish was used as experimental subjects to establish immune injury model of zebrafish induced by chloramphenicol and vinorelbine.To observe the effect of Buxue Shengsui Recipe on the number of neutrophils in zebrafish tail.The expression of interleukin 6(IL-6),interleukin 10(IL-10)and tumor necrosis factorα(TNF-α)mRNA was detected by Real time-PCR.Results Compared with the model group,0.5 mg·mL^(-1)and 2 mg·mL^(-1)Buxue Shengsui Recipe significantly increased the average strength of zebrafish dorsal blood vessels(P<0.01)in a dose-dependent manner.2 mg·mL^(-1)Buxue Shengsui Recipe significantly reversed the decrease of neutrophils caused by chloramphenicol and vinorelbine(P<0.01).Compared with the model group,the expression of IL-6 and IL-10 mRNA of zebrafish in Buxue Shengsui formula group was significantly increased(P<0.05 or P<0.01),and TNF-αmRNA expression was significantly decreased(P<0.05).Conclusion Buxue Shengsui Recipe can improve the anemia state of zebrafish induced by phenylhydrazine,and has obvious protective effect on the immune injury of zebrafish induced by chloramphenicol and vinorelbine,which is related to the upregulation of cytokines IL-6,IL-10 and the down-regulation of TNF-α.

关 键 词：补血生髓方 贫血 免疫损伤 斑马鱼 红细胞 中性粒细胞 

分 类 号：R285.5[医药卫生—中药学]