金缕半枫荷乙酸乙酯部位化学成分研究  

Chemical Constituents of Ethyl Acetate Extracts from Stirps of Semiliquidambar cathayensis

作　　者：徐宇 张昊祯 Muhammad Ishaq 吴嘉佳 王文沛 李莎莎 肖雪 严诗楷[1,4] 金慧子 Xu Yu;Zhang Haozhen;Ishaq Muhammad;Wu Jiajia;Wang Wenpei;Li Shasha;Xiao Xue;Yan Shikai;Jin Huizi(School of Pharmacy,Shanghai Jiao Tong University,Shanghai 200240,China;NMPA Key Laboratory for Rapid Testing Technology of Drugs,Guangdong Institute for Drug Control,Guangzhou 510663,China;The Second Affiliated Hospital of Guangzhou University of Chinese Medicine,Guangzhou 510006,China;Institute of Chinese Medicinal Sciences,Guangdong Pharmaceutical University,Guangzhou 510006,China)

机构地区：[1]上海交通大学药学院,上海200240 [2]国家药品监督管理局药品快速检验技术重点实验室(广东省药品检验所),广州510663 [3]广州中医药大学第二附属医院,广州510006 [4]广东药科大学中医药研究院,广州510006

出　　处：《世界科学技术-中医药现代化》2023年第11期3497-3505,共9页Modernization of Traditional Chinese Medicine and Materia Medica-World Science and Technology

基　　金：国家药品监督管理局快速检验技术重点实验室开放课题(KF2022002):基于离子淌度-高分辨质谱的岭南珍稀药材活性成分发现与鉴定,负责人:李莎莎;国家药品监督管理局快速检验技术重点实验室开放课题(KF2022006):基于Q-marker理论与近红外光谱技术的岭南特色药材快速质量评价研究,负责人:肖雪

摘　　要：目的研究金缕半枫荷茎枝乙酸乙酯提取部位的化学成分。方法采用硅胶、Sephadex LH-20柱色谱、高效液相色谱(HPLC)、高效液相制备色谱等将乙酸乙酯部位进行分离和纯化,波谱法鉴定化合物结构。对半枫荷总提、各提取部位和分离得到的14个化合物进行抗氧化活性测试。结果鉴定出14个化合物,分别为:肉豆蔻酸(1)、硬脂酸(2)、芝麻素(3)、9-十八碳烯酸(4)、亚油酸(5)、邻苯二甲酸二丁酯(6)、豆甾醇(7)、β-谷甾醇(8)、羽扇豆醇(9)、齐墩果酸(10)、lup-20(29)-ene-3-[3-keto-hexadecanoate](11)、防葵素(12)、C-藜芦酰乙二醇(13)、2,3-二羟基-1-(4-羟基-3,5-二甲氧基苯基)-1-丙酮(14)。结论首次通过分离手段从该植物的茎枝中得到11个化合物,分别为1、3-7、9、11-14,并且体外抗氧化实验表明半枫荷乙酸乙酯部位、分离纯化得到的化合物13和14抗氧化活性较强。Objective To study the chemical constituents from ethyl acetate extracts of the strips of Semiliquidambar cathayensis.Methods The strips of S.cathayensis were extracted by 80%ethanol and the extracts were evaporated.Fourteen compounds in ethyl acetate extracts were isolated and purified by various chromatographic techniques,such as silica gel,Sephadex LH-20 column and pre-HPLC,etc.Their structures were identified on the basis of physicochemical properties and spectroscopic analysis.Antioxidant activity test was used to evaluate total extraction,each extraction part and the isolates.Results Fourteen compounds were isolated from the strips of S.cathayensis and identified by NMR as tetradecanoic acid(1),stearic acid(2),sesamin(3),9-octadecenoic acid(4),linoleic acid(5),dibutylphthalate(6),stigmasterol(7),β-sitosterol(8),lupeol(9),oleanolic acid(10),lup-20(29)-ene-3-[3-keto-hexadecanoate](11),peujaponisin(12),C-veratroylglycol(13),and 2,3-dihydroxy-1-(4-hydroxy-3,5-dimethoxyphenyl)-1-propanone(14).Conclusion Compounds 1,3,4,5,6,7,9,11,12,13 and 14 were isolated from this plant for the first time.The EA part,compounds 13(C-veratroylglycol)and 14(2,3-dihydroxy-1-(4-hydroxy-3,5-dimethoxyphenyl)-1-propanone)showed significant antioxidative effects.

关 键 词：金缕半枫荷 乙酸乙酯部位 化学成分 结构鉴定 

分 类 号：R284.1[医药卫生—中药学]