电针次髎、耳甲调节大鼠胃内压和心率变异性效应方向一致性的实验研究  

Consistency Experiment of“Ciliao”(BL32)with Auricular Concha on Intragastric Pressure and Heart Rate Variability in Rats Driven by Electroacupuncture

作　　者：詹铮 潘彦宏 姜劲峰[1] Zhan Zheng;Pan Yanhong;Jiang Jinfeng(Key Laboratory of Acupuncture and Medicine Research of Ministry of Education,Nanjing University of Chinese Medicine,Nanjing 210023,China)

机构地区：[1]南京中医药大学针药结合教育部重点实验室,南京210023

出　　处：《世界科学技术-中医药现代化》2023年第7期2501-2508,共8页Modernization of Traditional Chinese Medicine and Materia Medica-World Science and Technology

基　　金：江苏省自然科学基金委员会面上项目(BK20221350):基于体表迷走神经刺激的耳甲电针抗炎自主神经环路机制研究,负责人:姜劲峰。

摘　　要：目的观察电针次髎与电针耳甲调节胃内压和心率变异性的效应方向一致性,并观察两者联合电针是否具有协同增效作用。方法SD雄性大鼠随机分为耳甲组、次髎组和“耳甲+次髎”组,每组8只。各组大鼠分别电针(30 Hz,2 mA,波宽0.2 ms±30%,连续波)2 min,并于电针前和电针期间检测胃内压、心率变异性。结果①与电针前相比,胃内压变化:耳甲电针干预可显著升高胃内压,尤其在干预的30-60 s(P<0.05)、60-90 s期间(P<0.05);次髎电针干预可显著升高胃内压,尤其在干预的60-90 s期间(P<0.05);“耳甲+次髎”电针干预可显著升高胃内压,尤其在干预的60-90 s(P<0.01)、90-120 s期间(P<0.05)。“耳甲+次髎”电针干预的胃内压变化倍率,既显著高于同期耳甲电针,尤其在干预的60-90 s(P<0.01)、90-120 s期间(P<0.01),也显著高于同期次髎电针,尤其在干预的60-90 s(P<0.05)、90-120 s期间(P<0.05)。②与电针前相比,心率变异性变化:耳甲电针干预的高频(HF)在干预全程均显著升高(P<0.01,P<0.05,P<0.01,P<0.05)、低频(LF)在干预全程均显著升高(P<0.01,P<0.01,P<0.05,P<0.05)、极低频(VLF)在干预全程均显著升高(P<0.05,P<0.05,P<0.05,P<0.05);次髎电针干预的HF在干预全程均显著升高(P<0.05,P<0.05,P<0.05,P<0.05)、LF在干预全程均显著升高(P<0.05,P<0.05,P<0.05,P<0.05)、VLF在干预全程均显著升高(P<0.05,P<0.05,P<0.05,P<0.05);“耳甲+次髎”电针干预的HF在干预全程均显著升高(P<0.05,P<0.05,P<0.05,P<0.05)、LF在干预全程均显著升高(P<0.001,P<0.05,P<0.01,P<0.01)、VLF在干预全程均显著升高(P<0.05,P<0.01,P<0.05,P<0.05)。耳甲电针干预可显著降低LF/HF,尤其在干预的0-30 s(P<0.01)、30-60 s(P<0.05)、60-90 s期间(P<0.05);次髎电针干预可显著降低LF/HF,尤其在干预的0-30 s期间(P<0.05);“耳甲+次髎”电针干预可显著降低LF/HF,尤其在干预的30-60 s(P<0.05)、60-90 s(P<0.01)、90-120 s期间(P<0.01)。“耳甲+次髎”电针干Objective To observe the consistency of the effect directions of electroacupuncture(EA)at the"Ciliao"(BL32)and EA on the auricular concha(AC)in regulating intragastric pressure(IGP)and heart rate variability(HRV),and to observe whether the combined EA of"Ciliao"(BL32)and AC has a synergistic effect.Methods SD male rats were divided into EA-AC group,EA-BL32 group and EA-"AC+BL32"group,with 8 rats in each group.And the IGP and HRV were measured before and during EA(30 Hz,2 mA,pluse width 0.2 ms±30%,continuous wave)for 2 min in each group.Results①Changes in IGP compared with before EA:the IGP in EA-AC group significantly increased especially during the 30th to 60th(P<0.05)and 60th to 90th second of EA(P<0.05);The IGP in EA-BL32 group significantly increased especially during the 60th to 90th second of EA(P<0.05);The IGP in EA-"AC+BL32"group significantly increased especially during the 60th to 90th(P<0.01)and 90th to 120th second of EA(P<0.05).The change rate of IGP of EA-"AC+BL32"group was significantly higher than that of the EA-AC group especially during the 60th to 90th(P<0.01)and 90th to 120th second of EA(P<0.01),and EA-BL32 group especially during the 60th to 90th(P<0.05)and 90th to 120th second of EA(P<0.05).②Changes in HRV compared with before EA:the high frequency(HF)significantly increased(P<0.01,P<0.05,P<0.01,P<0.05),low frequency(LF)significantly increased(P<0.01,P<0.01,P<0.05,P<0.05),and very low frequency(VLF)significantly increased(P<0.05,P<0.05,P<0.05,P<0.05)throughout the EA in EA-AC group;The HF significantly increased(P<0.05,P<0.05,P<0.05,P<0.05),LF significantly increased(P<0.05,P<0.05,P<0.05,P<0.05),and VLF significantly increased(P<0.05,P<0.05,P<0.05,P<0.05)throughout the EA in EA-BL32 group;The HF significantly increased(P<0.05,P<0.05,P<0.05,P<0.05),LF significantly increased(P<0.001,P<0.05,P<0.01,P<0.01),and VLF significantly increased(P<0.05,P<0.01,P<0.05,P<0.05)throughout the EA in EA-"AC+BL32"group.The LF/HF in EA-AC group significantly decreased especially during the 0 to 30th(P<0.0

关 键 词：胃内压 心率变异性 迷走神经调控 次髎 耳甲 

分 类 号：R245.97[医药卫生—针灸推拿学]