酸浆化学成分及抗胃溃疡活性研究  

Chemical Constituents of Physalis Alkekengi Linn. var. Franchetii and Its Anti-ulcer Activity

作　　者：王勇[1] 张炯怡 谢明军 宋晓宁 张志勇[1] 马莹[1] 李泽夏琼 赵丽[1] 邱红燕[1] 刘丹 甘甜 杨健 Wang Yong;Zhang Jiongyi;Xie Mingjun;Song Xiaoning;Zhang Zhiyong;Ma Ying;Li Zexiaqiong;Zhao Li;Qiu Hongyan;Liu Dan;Gan Tian;Yang Jian(Guizhou Provincial Institute for Food and Drug Control,Guiyang 550004,China;Guizhou Institute of Food Inspection&Testing,Guiyang 550004,China;Guizhou Medical Products Administration,Guiyang 550004,China)

机构地区：[1]贵州省食品药品检验所,贵阳550004 [2]贵州省食品检验检测院,贵阳550004 [3]贵州省药品监督管理局,贵阳550004

出　　处：《世界科学技术-中医药现代化》2022年第8期3043-3056,共14页Modernization of Traditional Chinese Medicine and Materia Medica-World Science and Technology

基　　金：贵州省科技厅科技支撑项目(黔科合SY字【2015】3032):贵州特色苗药喔嘎良、酸浆的成药性评价及丹参酮IIA微球、蜘蛛香总缬草三酯固体自微乳创新药研发,负责人:陶玲、刘亚华、王勇

摘　　要：目的明确酸浆中哪些成分具有抗胃溃疡活性。方法本课题组对酸浆乙酸乙酯萃取部位进行系统化学成分分离。酸浆乙酸乙酯部位在大孔吸附树脂富集后经过反复硅胶柱色谱进行分离,再根据理化性质和波谱数据鉴定化合物的结构。采用乙醇诱导胃溃疡模型测定粗分片段和目标化合物抗胃溃疡活性。结果从酸浆活性部位分离获得30个化合物,化合物3、4、12、24和30为首次从酸浆中分离得到。9组粗分片段中,Fr G、Fr K和Fr OPQR三组表现出显著的抗胃溃疡活性(P<0.05,P<0.01),抑制率分别为55.3%、74.5%和93.8%,与之相比较,法莫替丁组的抑制率为89.3%;灌胃剂量为6.25、12.5和25 mg·kg^(-1)时,酸浆苦味素B的抑制率分别为27.8%、67.3%和74.6%;灌胃剂量为10、20和40 mg·kg^(-1)时,木犀草苷的抑制率分别为29.7%、45.6%和64.2%。结论酸浆苦味素B和木犀草苷可能是酸浆抗胃溃疡活性的主要成分。Objective In order to identify which components of Physalis alkekengi Linn.var.Franchetii have anti-ulcer activity.Methods The ethyl acetate extract of Physalis alkekengi Linn.var.franchetii were isolated and studied systemically in chemistry.Compounds from EtOAc layer was enriched by AB-8 macro porous resin and isolated repeatedly by silica gel columns.On the basis of chemicophysical properties and spectral analysis,the structures of these compounds were identified.Segments and target compounds were evaluated for anti-ulcer activity using ethanolinduced gastric lesions test in rats.Results 30 known compounds were isolated from EAF,compounds 3、4、12、24and 30 are isolated from Physalis alkekengi Linn.var.franchetii for the first time.The result indicated that Fr G,Fr K and Fr OPQR showed significant anti-ulcer activity(P<0.05,P<0.01),the rate of inhibition were 55.3%,74.5%and93.8%,respectively.In contrast,the famotidine group was 89.3%.The inhibitory rates of physalin B were 27.8%、67.3%and 74.6%at the doses of 6.25,12.5 and 25 mg·kg^(-1),and the inhibitory rates of luteolin were 29.7%、45.6%and64.2%at the doses of 10,20 and 40 mg·kg^(-1),respectively.Conclusion It can be speculated that physalin B and luteolin might be the main ingredients of the effect of Physalis alkekengi Linn.var.franchetii on gastric ulcer.

关 键 词：酸浆 化学成分 苦味素B 木犀草苷 抗胃溃疡 

分 类 号：R284.1[医药卫生—中药学] R285[医药卫生—中医学]