虎杖RGF多肽的系统进化分析及其在根发育中的功能研究  

Phylogenetic Analysis of RGF Peptides in Polygonum Cuspidatum and Its Function in Root Development

作　　者：郑兰兰 李彤晖 肖潇 罗湘胤 张勇洪 甄伟波 孟昆 刘娟[4] 王国栋[5] 李琛 Zheng Lanlan;Li Tonghui;Xiao Xiao;Luo Xiangyin;Zhang Yonghong;Zhen Weibo;Meng Kun;Liu Juan;Wang Guodong;Li Chen(Laboratory of Medicinal Plant,Institute of Basic Medical Sciences,School of Basic Medicine,Biomedical Research Institute,Hubei Key Laboratory of Wudang Local Chinese Medicine Research,Hubei University of Medicine,Shiyan 442000,China;State Key Laboratory of Crop Stress Adaptation and Improvement,Henan University,Kaifeng 475001,China;Taihe Hospital Affiliated to Hubei University of Medicine,Shiyan 442000,China;National Resource Center for Chinese Materia Medica,China Academy of Chinese Medical Sciences,Beijing 100700,China;Key Laboratory of Medicinal Resources and Natural Pharmaceutical Chemistry,Ministry of Education,National Engineering Laboratory for Resource Development of Endangered Crude Drugs in Northwest China,College of Life Sciences,Shaanxi Normal University,Xi'an 710119,China)

机构地区：[1]湖北医药学院基础医学院药用植物实验室/生物医药研究院/武当特色中药研究湖北省重点实验室,十堰442000 [2]河南大学作物逆境适应与改良国家重点实验室,开封475001 [3]湖北医药学院附属太和医院,十堰442000 [4]中国中医科学院中药资源中心,北京100700 [5]陕西师范大学生命科学学院/西北濒危药材资源开发国家工程实验室/药用资源与天然药物化学教育部重点实验室,西安710119

出　　处：《世界科学技术-中医药现代化》2022年第7期2684-2694,共11页Modernization of Traditional Chinese Medicine and Materia Medica-World Science and Technology

基　　金：湖北省卫生健康委员会中医药青年项目(ZY2019Q004):秦巴片区特色药用植物虎杖的本草基因组学研究,负责人:李琛;中国中医科学院中央本级重大增减支项目“名贵中药资源可持续利用能力建设项目”(2060302):虎杖中活性小分子S7调控侧根干细胞重编程的机制研究,负责人:李琛;湖北省科学技术厅湖北重点实验室建设基金(WLSP201905):武陵山区特色药用植物虎杖的代谢调控研究,负责人:李琛

摘　　要：目的鉴定与分析了虎杖中RGF多肽序列特征,阐明虎杖RGF多肽对植物根型的调控作用,为虎杖优形优质新品种奠定了理论研究基础。方法利用生物信息学的方法鉴定了虎杖RGF编码基因,并对其基序进行了比对、偏好性分析及系统进化分析。利用模式植物拟南芥根发育的体系,研究了虎杖PcRGF对根发育的影响。结果从虎杖基因组中鉴定出5个RGF基因(PcRGF1-5),系统进化分析表明PcRGF2-5与拟南芥AtRGF1、AtRGF4的亲缘关系较近,聚类为一族。PcRGF1与其他虎杖PcRGF较为分散,同拟南芥AtRGF9、AtRGF6的亲缘关系较近。与对照组相比,PcRGF2及PcRGF5影响根的重力反应,抑制了根的波浪(waving)表型。PcRGF4处理后主根长度相对于对照组显著增加,表明其促进根的生长。结论揭示了虎杖RGF家族的序列特征和系统进化关系,初步探究了虎杖PcRGF的生物学功能,发现虎杖PcRGF具备调控根发育的保守功能。Objective To identify RGF peptides in Polygonum cuspidatum and analyze its phylogenetic tree,and investigate the function of PcRGF in root development,which will provide the basis for molecular breeding of this medicinal plant.Methods By using bioinformatics approaches,RGF genes were identified in Polygonum cuspidatum.RGF motif,sequence logos and phylogenetic tree were analyzed.The PcRGF function was analyzed based on Arabidopsis root system.Results 5 RGF genes were identified in Polygonum cuspidatum.Phylogenetic analysis showed that PcRGF2-5 were clustered together and close to AtRGF1 and AtRGF4.However,PcRGF1 was not clustered with other PcRGF and closer to AtRGF9 and AtRGF6.PcRGF2 and PcRGF5 altered root gravity response and inhibited root waving phenotype.PcRGF4 promoted Arabidopsis root growth as compared with mock.Conclusion This paper reveals the sequences feature and phylogenetic relationship of PcRGF,investigates the function of PcRGF in root development,which the roots of Polygonum cuspidatum are designed as medical use.

关 键 词：虎杖 多肽激素 根分生区生长因子 根 拟南芥 

分 类 号：S567.239[农业科学—中草药栽培]