基于分子印迹技术分离淡竹叶中异荭草苷的研究  

Extraction of Isoorientin in Lophatherum Gracile Based on Molecular Imprinting Technique

作　　者：朱俊访[1] 李博[1] 聂阳[1] Zhu Junfang;Li Bo;Nie Yang(Guangdong Food and Drug Vocational College,Guangzhou 510520,China)

机构地区：[1]广东食品药品职业学院实验实训中心,广州510520

出　　处：《世界科学技术-中医药现代化》2020年第8期2799-2806,共8页Modernization of Traditional Chinese Medicine and Materia Medica-World Science and Technology

基　　金：广东食品药品职业学院自然科学研究项目(2017ZR027):利用分子印迹技术定向分离淡竹叶中黄酮类成分及活性研究,负责人:李博

摘　　要：目的利用分子印迹技术,建立一种制备异荭草苷分子印迹聚合物的方法,并将其用于中药淡竹叶中异荭草苷的分离研究。方法本研究以异荭草苷为模板分子,2-乙烯基吡啶为功能单体,乙二醇二甲基丙烯酸酯为交联剂,通过沉淀聚合法制备印迹聚合物,对聚合物表面形态进行表征,研究聚合物的吸附与分离性能。结果制备所得印迹聚合物对异荭草苷具有吸附能力和一定的分离能力,最大吸附量可达2.5μmol·g-1。结论分子印迹聚合物可用于淡竹叶中异荭草苷的分离。Objective Using the molecular imprinting technique,to establish a method for preparing the molecularly imprinted polymers(MIPs)of Isoorientin,and studying on the extraction of Isoorientin in Lophathemm gracile.Methods In this study,Isoorientin was used as template,2-Vinylpyridine was used as functional monmer,and ehtylene glycol dimethacrylate was used as a cross-linking agent.Using the precipitation polymerization to prepare the molecularly imprinted polymer.The characterization of the obtained MIPs was evaluated by scanning electron microscope.A test was conducted to investigate the performance of adsorotion and extraction.Results The experiment showed that the molecularly imprinted polymers has certain adsorption property with Isoorientin and the adsorption capacity up to2.5μmol·g-1.Conclusion The molecularly imprinted polymers can be used to extract Isoorientin in Lophathemm gracile.

关 键 词：分子印迹技术 印迹聚合物 淡竹叶 异荭草苷 

分 类 号：R284.1[医药卫生—中药学]