葫芦茶苷体内抗鸭乙型肝炎病毒及保肝作用研究  

Anti-viral and Hepato-protective Effects of Tadehaginoside in Duck Infected with Hepatitis B Virus

作　　者：唐爱存[1,2] 卢秋玉 韦燕飞[4] 谢海源[4] 刘喜华[5] Tang Aicun;Lu Qiuyu;Wei Yanfei;Xie Haiyuan;Liu Xihua(The First Affiliated Hospital of Guangxi Traditional Chinese Medical University,Nanning 530023,China;GuangXi Zhuang Yao Medicine Centrer of Engineering and Technology,Nanning 530200,China;People’s Hospital of Guangxi Zhuang Autonomous Region,Nanning 530021,China;Guangxi University of Chinese Medical,Nanning 530001,China;Guangxi Medical College,Nanning 530023,China)

机构地区：[1]广西中医药大学第一附属医院,南宁530023 [2]广西壮瑶药技术研究中心,南宁530200 [3]广西壮族自治区人民医院,南宁530021 [4]广西中医药大学,南宁530001 [5]广西卫生职业技术学院,南宁530023

出　　处：《世界科学技术-中医药现代化》2020年第4期1096-1101,共6页Modernization of Traditional Chinese Medicine and Materia Medica-World Science and Technology

基　　金：广西壮族自治区科学技术厅广西自然科学基金青年基金项目(2015GXNSFBA139126):壮药葫芦茶中一种新化合物-葫芦茶苷抗乙型肝炎病毒的作用及其机制研究,负责人:唐爱存;广西卫生计生委研究项目(Z2015481):葫芦茶苷调控JAK/STAT信号通路抗乙型肝炎病毒的初步研究,负责人:唐爱存;广西中医药大学第一附属医院院内制剂研究与开发(2018ZJ002):参杞强精丸的制剂研究,负责人:唐爱存

摘　　要：目的考察葫芦茶苷对鸭乙型肝炎病毒(hepatitis B virus,HBV)的体内抑制作用及其保肝作用。方法以广西麻鸭为实验动物,建立感染鸭乙肝病毒的鸭乙肝动物模型,随机分为模型组、拉米夫定阳性对照组和葫芦茶苷低、中、高剂量组,每组10只,每日灌胃给药1次,连续给药14天,模型组每天灌服等体积的生理盐水。采用ELISA法检测鸭血清乙型肝炎表面抗原(DHBsAg)和鸭血清乙型肝炎E抗原(DHBeAg)的含量。采用实时荧光定量PCR法检测鸭血清鸭乙肝病毒DNA的含量。并检测鸭血清谷丙转氨酶(ALT)和谷草转氨酶(AST)的变化,检测肝组织匀浆超氧化物歧化酶(SOD)、谷胱甘肽过氧化物酶(GSH-PX)活性和还原型谷胱甘肽(GSH)、丙二醛(MDA)的含量。苏木精-伊红染色法(HE)观察鸭肝组织病理学变化。结果与模型组比较,葫芦茶苷各剂量组能显著降低血清DHBsAg和DHBeAg的滴度以及DHBV DNA的含量(P<0.05或P<0.01)。而且能降低鸭血清ALT、AST活性和MDA含量,增加肝组织SOD、GSH-PX活性和GSH含量。病理学检查结果提示葫芦茶苷能显著减轻肝细胞损伤程度。结论葫芦茶苷对鸭乙型肝炎病毒具有体内抑制作用,对DHBV引起肝损伤具有保护作用。Objective To investigate the inhibitory effect of tadehaginoside(TA)on duck hepatitis B virus and its liver protection.Methods The Guangxi brown spot ducklings were used as experimental animal,to establish an animal model of duck hepatitis b virus infected by DHBV,and randomly divided into six groups,model group,positive control group(lamivudine),TA low-dose,middle-dose and high-dose group,with 10 ducks in each group.The experimental ducks were administrated with drugs per oral for 14 days,once a day,and the model group were given normal saline.The titers of DHBsAg and DHBeAg were determined by ELISA,the quantity of DHBV DNA in serum was assayed by FQPCR,The activities of serum ALT and AST were detected,the activity of SOD and GSH-PX in hepatical tissues were measured,and the contents of GSH and MDA in liver homogenate were also determined.Histopathological changes of duck liver were observed by HE staining.Results Compared with the model group,TA could significantly reduce the serum DHBV DNA,DHBsAg and DHBeAg(P<0.05 or P<0.01).The activities of ALT,AST,and MDA were obviously decreased,however,the activities of SOD,GSH-PX and GSH were obviously increased(P<0.05 or P<0.01).The results of pathological examination indicated that TA could significantly reduce the degree of hepatocyte injury.Conclusion Tadehaginoside can inhibit replication of DHBV,and protect liver against injury caused by DHBV.

关 键 词：葫芦茶苷 鸭乙型肝炎病毒 体内实验 保肝作用 

分 类 号：R259[医药卫生—中西医结合]