小檗碱对幽门螺杆菌感染大鼠胃黏膜IFN-γ、TNF-α、IL-2表达的影响  

Effects of berberine on expressions of IFN-γ, TNF-α and IL-2 in gastric mucosa of rats infected with Helicobacter pylori

作　　者：郭丽坤[1] 岑戎[1] GUO Likun;CEN Rong(Endoscopy Center,Shuguang Hospital Affiliated to Shanghai University of Traditional Chinese Medicine,Shanghai 200021,China)

机构地区：[1]上海中医药大学附属曙光医院内镜中心,上海200021

出　　处：《上海中医药杂志》2020年第S01期159-161,166,共4页Shanghai Journal of Traditional Chinese Medicine

摘　　要：目的研究小檗碱对幽门螺杆菌所致胃炎模型大鼠胃黏膜IFN-γ、TNF-α、IL-2表达的影响。方法采用Hp标准株SS 2000用灌胃方法建立幽门螺杆菌胃炎大鼠模型,分为空白组、模型组、小檗碱组,灌胃结束4周后处死大鼠取胃窦部黏膜,采用HE染色观察胃黏膜炎症情况,应用qRT-PCR、Western blot法检测大鼠胃黏膜IFN-γ、TNF-α、IL-2基因的mRNA和蛋白表达情况。结果①小檗碱作用后,与模型组相比,小檗碱组胃黏膜中的炎性细胞浸润明显减少,提示小檗碱可改善幽门螺杆菌感染引起的胃黏膜炎症。②经小檗碱干预后,胃黏膜组织的IFN-γ、TNF-α、IL-2 mRNA水平较模型组下调(P<0.05),但与空白组依然有明显差异(P<0.05)。③Western blot结果显示,模型组大鼠胃黏膜内IFN-γ、TNF-α、IL-2基因蛋白表达增加,小檗碱组较模型组IFN-γ、TNF-α、IL-2基因蛋白均有降低。结论小檗碱可能通过调节IFN-γ、TNF-α、IL-2的表达,减轻幽门螺杆菌所致胃炎的胃黏膜炎症。Objective To study the effects of berberine on the expressions of IFN-γ,TNF-αand IL-2 in gastric mucosa of gastritis rats induced by Helicobacter pylori(Hp).Methods Hp standard strain SS 2000 with intragastric administration was used to establish the rat model of Helicobacter pylori gastritis,and the rats was divided into the blank group,model group and berberine group.After intragastric administration for 4 weeks,the rats were sacrificed to collect the gastric antrum mucosa.The inflammation of gastric mucosa was observed by HE staining.The mRNA and protein expressions of IFN-γ,TNF-α,IL-2 in the gastric mucosa of rats were detected by qRT-PCR and Western blot.Results①After berberine treatment,compared with the model group,the inflammatory cell infiltration in the gastric mucosa of the berberine group was significantly reduced,suggesting that berberine could improve the inflammation of gastric mucosa caused by Hp infection.②After berberine intervention,the mRNA levels of IFN-γ,TNF-αand IL-2 in gastric mucosa were lower than those in the model group(P<0.05),but there were still statistically significant differences between the berberine group and the blank group(P<0.05).③The results of Western blot showed that the protein expressions of IFN-γ,TNF-αand IL-2 in the gastric mucosa of the model group were increased,while the protein expressions of IFN-γ,TNF-αand IL-2 in the berberine group were decreased compared with the model group.Conclusion Berberine may alleviate the inflammation of gastric mucosa caused by Helicobacter pylori by regulating the expressions of IFN-γ,TNF-αand IL-2.

关 键 词：幽门螺杆菌 小檗碱 IFN-Γ TNF-α IL-2 

分 类 号：R285.5[医药卫生—中药学]