淫羊藿对光损伤小鼠视网膜光感受器细胞的保护作用  

Protective effects of Epimedium on photoreceptor cells in light-damaged mouse retinas

作　　者：马荣 吴晗晗 郭红 杜霄烨 徐静 崔金刚[1,2] 张腾 陈瑜[1,2] MA Rong;WU Hanhan;GUO Hong;DU Xiaoye;XU Jing;CUI Jingang;ZHANG Teng;CHEN Yu(Yueyang Hospital of Integrative Chinese and Western Medicine Affiliated to Shanghai University of Traditional Chinese Medicine,Shanghai 200437,China;Clinical Research Department of Integrated Traditional Chinese and Western Medicine,Shanghai Institute of Traditional Chinese Medicine,Shanghai 200437,China)

机构地区：[1]上海中医药大学附属岳阳中西医结合医院中西医结合临床研究所,上海200437 [2]上海市中医药研究院中西医结合临床研究所,上海200437

出　　处：《上海中医药杂志》2020年第2期91-96,共6页Shanghai Journal of Traditional Chinese Medicine

基　　金：国家自然科学基金面上项目(81673790,81903887);上海市优秀学术带头人项目(19XD1403700);上海市东方学者跟踪计划项目(GZ2017064).

摘　　要：目的研究淫羊藿对光感受器细胞的保护作用,为淫羊藿配伍应用治疗相关视网膜退行性疾病提供药效学依据。方法将雌性BALB/c小鼠随机分为未光照组(No light)、光照组(light vehicle)、淫羊藿干预组(Epimedium),每组6只。采用强光照射的方法制备小鼠光感受器细胞退行性病变模型,以临床常用剂量淫羊藿水煎液腹腔注射小鼠2次。采用光学相干断层扫描技术分析视网膜结构,采用视网膜电图分析视网膜功能,采用免疫组织化学的方法分别分析光感受器细胞视紫红质(rhodopsin)和视蛋白M(opsin-M)、双极细胞蛋白激酶Cα(PKC-α)、水平细胞钙结合蛋白D(calbindin-D)和神经胶质细胞胶质纤维酸性蛋白(GFAP)的表达;采用TUNEL法分析视网膜细胞凋亡情况。结果光照后,小鼠视网膜外核层缺失,视网膜功能受损;视网膜Rhodopsin、Opsin-M、PKC-α、Calbindin-D和GFAP等蛋白表达缺失或异常;外核层出现大量TUNEL阳性细胞。淫羊藿干预的小鼠视网膜结构完整、功能正常;上述蛋白与未光照小鼠相比,未见异常表达;视网膜外核层未见细胞凋亡。结论淫羊藿对光损伤引起的光感受器细胞退行性改变具有显著的治疗作用。Objective To observe the protective effects of Epimedium on photoreceptor cells and to provide a pharmacodynamic basis for Epimedium that used with other drugs to treat retinal degenerative diseases in clinic.Methods Female Balb/C mice were randomly divided into three groups:no light group,light vehicle group and Epimedium intervention group,6 in each group.A mouse model of photoreceptor cell degeneration was prepared by light injury,and the decoction of Epimedium was given twice to the light-injured mice by intraperitoneal injection before and after the light injury.The retinal structure was examined by optical coherence tomography,and the retinal function was analyzed by electroretinogram.Retinal histopathological sections were prepared to analyze the expression of rhodopsin,opsin-M,PKC-α,calbindin-D and GFAP in retina,respectively,and TUNEL assay was conducted to analyze apoptosis of retinal cells.Results After the light exposure,the outer nuclear layer(ONL)of the retina was absent and the retinal function was impaired;the expression of retinal protein including rhodopsin,opsin-M,PKC-α,calbindin-D and GFAP was absent or abnormal,respectively;and a large number of TUNEL positive cells were observed in the ONL.Among Epimedium-treated mice,the structure of mouse retina was intact with proper function,the expressions of above proteins were normal compared with no-light injured mice,and the retinal apoptosis was not observed in the ONL.Conclusion Epimedium has a significant therapeutic effect on the photoreceptor cell degeneration induced by light injury.

关 键 词：淫羊藿 视网膜退行性病变 光感受器细胞 细胞凋亡 小鼠 

分 类 号：R285.5[医药卫生—中药学]