右归丸对糖尿病性勃起功能障碍大鼠海绵体组织氧化应激的影响  

Effect of Yougui Pill on oxidative stress of corpus cavernosum tissue in rats with diabetic erectile dysfunction

作　　者：翟新宇[1] 谈鸣岳 葛旻垚[1] 万众 徐东亮 ZHAI Xinyu;TAN Mingyue;GE Minyao;WAN Zhong;XU Dongliang(Urology Center,Shuguang Hospital Affiliated to Shanghai University of Traditional Chinese Medicine,Shanghai 201203,China)

机构地区：[1]上海中医药大学附属曙光医院泌尿中心,上海201203

出　　处：《上海中医药大学学报》2022年第S01期227-231,共5页Academic Journal of Shanghai University of Traditional Chinese Medicine

基　　金：上海中医药大学预算内基金项目(2021LK077)

摘　　要：目的:通过观察右归丸对糖尿病性勃起功能障碍(DED)大鼠阴茎海绵体组织氧化应激的影响,探讨右归丸治疗DED的作用机制。方法:成熟的雄性SD大鼠腹腔注射链脲佐菌素(STZ)构建大鼠糖尿病模型,阿扑吗啡(APO)试验观察大鼠勃起功能情况并筛选DED模型。将DED大鼠分成模型组(10只)、右归丸组(10只),并设雄性SD大鼠10只为空白对照组。给药8周后,阿扑吗啡试验观察大鼠的勃起状况;取大鼠尾静脉血测定葡萄糖值水平;取大鼠的阴茎海绵体组织,测定丙二醛(MDA)浓度、超氧化物歧化酶(SOD)和谷胱甘肽还原酶(GSH-Px)活性,TUNEL染色测定海绵体组织内皮细胞的凋亡状况;Western blot测定核因子E2相关因子(Nrf2)和血红素加氧酶-1(HO-1)的蛋白表达水平。结果:①与对照组比较,模型组血糖水平升高,勃起功能下降,抗氧化能力减轻,细胞凋亡增加;②与模型组比较,右归丸组总勃起次数增加,勃起潜伏时间减少,血糖水平无明显差异,但MDA含量明显降低(P<0.05),SOD和GSH-Px活力以及Nrf2和HO-1蛋白表达明显升高(P<0.05),细胞凋亡减少。结论:右归丸可能通过激活Nrf2-ARE信号通路降低DED大鼠氧化应激损伤,减少海绵体内皮细胞凋亡,从而改善糖尿病性勃起功能障碍。Objective:To explore the mechanism of Yougui Pill in the treatment of DED by observing the effect of Yougui Pill on oxidative stress in corpus cavernosum tissue of diabetic erectile dysfunction(DED)rats.Method:The diabetic rat model was established by intraperitoneal injection of streptozotocin(STZ)in mature male SD rats.The erectile function of rats was observed by apomorphine(APO)test and the DED model was screened.DED rats were divided into model group(n=10)and Yougui Pill group(n=10),and 10 of the most robust male SD rats were selected as control group.Eight weeks after administration,the erection of rats was observed by apomorphine test,the blood of tail vein was taken to determine the level of glucose.The corpus cavernosum tissue of rats were taken,of which the concentration of malondialdehyde(MDA),the activity of superoxide dismutase(SOD)and glutathione reductase(GSH-Px)were determined,and the apoptosis of endothelial cells were determined by TUNEL staining.The protein expression levels of nuclear erythroid 2-related factor 2(Nrf2)and heme oxygenase-1(HO-1)were measured by Western blot.Result:①Compared with the control group,the blood glucose level increased,the erectile function decreased,the antioxidant capacity decreased,and the cell apoptosis increased in the model group.②Compared with the model group,in Yougui Pill group,the total number of erections increased,the erectile latent time decreased,and the blood glucose level had no significant difference,but the content of MDA decreased significantly(P<0.05),the activity of SOD and GSH-Px as well as the expression of Nrf2 and HO-1 protein increased significantly(P<0.05),and the cell apoptosis decreased.Conclusion:Yougui Pill may reduce oxidative stress injury and apoptosis of corpus cavernosum endothelial cells in DED rats by activating Nrf2-ARE signal pathway,thus improving diabetic erectile dysfunction.

关 键 词：右归丸 糖尿病性勃起功能障碍 氧化应激 细胞凋亡 Nrf2-ARE 

分 类 号：R285.5[医药卫生—中药学]