丝素纤维人工韧带材料的表面改性及细胞相容性  

Surface Modification and Cytocompatibility of Silk Fibroin Fiber Artificial Ligament Materials

作　　者：王一婷 刘明洁 王璐[1] 关国平[1] WANG Yiting;LIU Mingjie;WANG Lu;GUAN Guoping(Key Laboratory of Textile Science&Technology of Ministry of Education,College of Textiles,Donghua University Shanghai,201620)

机构地区：[1]东华大学纺织学院纺织面料技术教育部重点实验室,上海201620

出　　处：《生物医学工程学进展》2020年第3期125-129,共5页Progress in Biomedical Engineering

基　　金：科技部国家重点研发计划项目:(2018YFC1106200,2018YFC1106201)

摘　　要：该文研究设计并制备了一种可降解的丝素纤维人工韧带材料,并对其进行了表面改性和细胞相容性研究。以丝素纤维为原材料编织仿生结构的人工韧带,对其材料表面进行低温氧等离子体处理,再通过EDC/NHS接枝I型胶原蛋白。同法接枝人纤维蛋白原和转化因子TGF-β1结合肽到材料表面。对材料的细胞相容性进行评价,结果表明,经过等离子体处理和生物活性分子接枝后,细胞相容性有了进一步提升。成纤维细胞在接枝了人纤维蛋白原的材料表面活性更高。A biodegradable silk fibroin fiber artificial ligament prototype was designed and fabricated in the present work.Surface modification was further carried out and cytocompatibility of the modified prototypes was evaluated.Firstly,silk fibroin was used as raw material to weave artificial ligament with bionic structure.Moreover,oxygen plasma and EDC/NHS were employed to treat the prototype and to immobilize type I collagen(Col I),human fibrinogen(Fg)and TGF-β1 binding peptide(Pep)onto the surfaces.Furthermore,the cytocompatibility of the prototypes was evaluated by CCK-8 assay.The results showed that the cellular compatibility was improved following the plasma treatment and biomolecule grafting,and the viability of fibroblasts on the Fg-grafted samples showed significantly higher than those on other materials.

关 键 词：丝素纤维 人工韧带 表面改性 细胞相容性 可降解 

分 类 号：R318[医药卫生—生物医学工程] TP391[医药卫生—基础医学]