人B细胞永生化研究进展  

Advances in immortalization of human B cells

作　　者：钟周玥 于思远 葛胜祥[1,2] Zhouyue Zhong;Siyuan Yu;Shengxiang Ge(School of Public Health,Xiamen University,Xiamen 361102,Fujian,China;National Institute of Diagnostics and Vaccine Development in Infectious Diseases(Xiamen University),Xiamen 361102,Fujian,China)

机构地区：[1]厦门大学公,共卫生学院,福建厦门361102 [2]国家传染病诊断试剂与疫苗工程技术研究中心(厦门大学),福建厦门361102

出　　处：《生物工程学报》2021年第1期30-39,共10页Chinese Journal of Biotechnology

基　　金：国家传染病重大专项(No.2017ZX10302201-002-003);福建省自然科学基金(No.2019J02004)资助

摘　　要：人源单克隆抗体具有免疫原性低、半衰期长等优势,成为了体内应用中不可或缺的生物制剂。人类抗体库为人源单克隆抗体的制备提供了丰富的来源,人B细胞永生化是获得人类抗体库的潜在有效方法,可应用于人源单克隆抗体的制备。由于各平台均有亟待解决的问题,基于人B细胞永生化的抗体制备尚局限在实验室研究阶段,且目前尚缺乏一篇系统综述以明确现有的人B细胞永生化抗体制备平台的优劣及其可行性分析。因此文中就基于人B细胞永生化方法制备人源单克隆抗体的研究展开综述,以期为人源单克隆抗体制备技术的进一步发展提供参考。With the advantages of low immunogenicity and long half-life,human monoclonal antibody has become an indispensable biological agent in vivo.Immortalization of human B cells is a potential and effective method to obtain natural human antibody library,which can provide a rich source for the preparation of human monoclonal antibodies.As there are urgent problems to be solved in each platform,the preparation of antibodies based on human B cell immortalization is still limited to the laboratory research stage.At present,there is a lack of a systematic review to clarify the advantages and disadvantages of the existing human B cell immortalization antibody preparation platform and its feasibility analysis.This paper reviews the research on the preparation of human monoclonal antibody based on human B cells immortalization,and describes an in vitro cell culture method,in which hCD40L vesicles are used instead of feeder cells,in order to provide references for the further development of human monoclonal antibody preparation technology.

关 键 词：B细胞永生化 人源单克隆抗体 体外诱导培养 囊泡 

分 类 号：R392[医药卫生—免疫学]