The complete mitochondrial genome of the hybrid grouper Epinephelus moara(♀)×Epinephelus tukula(♂), and phylogenetic analysis in subfamily Epinephelinae  

作　　者：Zhentong Li Yongsheng Tian Meiling Cheng Linna Wang Jingjing Zhang Yuping Wu Zunfang Pang Wenhui Ma Jieming Zhai 

机构地区：[1]College of Fisheries and Life Science,Shanghai Ocean University,Shanghai 201306,China [2]Laboratory for Marine Fisheries Science and Food Production Processes,Pilot National Laboratory for Marine Science and Technology(Qingdao),Qingdao 266071,China [3]Yellow Sea Fisheries Research Institute,Chinese Academy of Fishery Sciences,Qingdao 266071,China [4]College of Fisheries and Life Science,Dalian Ocean University,Dalian 116023,China [5]Laizhou Mingbo Aquatic Co.Ltd.,Laizhou 261400,China

出　　处：《Acta Oceanologica Sinica》2020年第12期65-75,共11页海洋学报（英文版）

基　　金：The Key Research and Development Program of Shandong Province under contact No.2019GHY112063;the Breeding Project of Shandong Province under contract No.2019LZGC020;the Central Public-interest Scientific Institution Basal Research Fund Chinese Academy of Fishery Sciences under contract Nos 2020XT0601,2020TD19 and 2020TD25;the Yellow Sea Fisheries Research Institute Research Fees under contract Nos 20603022019002 and 20603022020015

摘　　要：The mitochondrial genome(mitogenome)of hybrid grouper Epinephelus moara(♀)×Epinephelus tukula(♂),a new hybrid progeny,can provide valuable information for analyzing phylogeny and molecular evolution.In this study,the mitogenome was analyzed using PCR amplification and sequenced,then the phylogenetic relationship of E.moara(♀)×E.tukula(♂)and 35 other species were constructed using Maximum Likelihood and NeighborJoining methods with the nucleotide sequences of 13 conserved protein-coding genes(PCGs).The complete mitogenome of E.moara(♀)×E.tukula(♂)was 16695 bp in length,which contained 13 PCGs,2 rRNA genes,22 tRNA genes,a replication origin and a control region.The composition and order of these genes were consistent with most other vertebrates.Of the 13 PCGs,12 PCGs were encoded on the heavy strand,and ND6 was encoded on the light strand.The mitogenome of the E.moara(♀)×E.tukula(♂)had a higher AT nucleotide content,a positive AT-skew and a negative GC-skew.All protein initiation codons were ATG,except for COX and ND4(GTG),ATP6(CTG),and ND3(ATA).ND2,COXII,ND3,ND4 and Cytb had T as the terminating codon,COXIII’s termination codon was TA,and the remaining PCGs of that were TAA.All tRNA genes,except for the lacking DHU-arm of tRNASer(AGN),were predicted to form a typical cloverleaf secondary structure.In addition,sequence similarity analysis(99%identity)and phylogenetic analysis(100%bootstrap value)indicated that the mitochondrial genome was maternally inherited.This study provides mitogenome data for studying genetic,phylogenetic relationships and breeding of grouper.

关 键 词：Epinephelus moara(♀)×Epinephelus tukula(♂) Epinephelus moara Epinephelus tukula mitochondrial genome PHYLOGENY 

分 类 号：S917.4[农业科学—水产科学]