利用时间质谱检出312例6月龄内健康婴儿鼻咽部微生态菌群的分布特征  

Distribution characteristics of nasopharyngeal microbiota of 312 healthy infants under 6 months of age detected by MALDI-TOF MS

作　　者：关恒云[1] 刘岚铮[1] 姜慧钰 白爱英[1] 刘辉[1] 赵小冬[2] 高尚[3] 李娜 阮师漫[1] GUAN Hengyun;LIU Lanzheng;JIANG Huiyu;BAI Aiying;LIU Hui;ZHAO Xiaodong;GAO Shang;LI Na;RUAN Shiman(Bacterial Disease Inspection Laboratory,Jinan Center for Disease Control and Prevention,Jinan 250021,Shandong,China;Immunology Institute,Jinan Center for Disease Control and Prevention,Jinan 250021,Shandong,China;Institute for Infectious Diseases Control,Jinan Center for Disease Control and Prevention,Jinan 250021,Shandong,China)

机构地区：[1]济南市疾病预防控制中心细菌性疾病检验所,山东济南250021 [2]济南市疾病预防控制中心免疫所,山东济南250021 [3]济南市疾病预防控制中心传染病所,山东济南250021

出　　处：《山东大学学报（医学版）》2022年第10期49-56,共8页Journal of Shandong University:Health Sciences

基　　金：传染病预防控制国家重点实验室自主研究开放课题(2019SKLID310,2020SKLID307);济南市卫生健康委员会科技计划项目(2020-4-105)

摘　　要：目的利用基质辅助激光解吸电离飞行时间质谱(MALDI-TOF MS)技术研究济南地区6月龄内健康婴儿鼻咽部微生态菌群分布特征,为鼻咽部微生态研究提供样本库信息。方法采集2019年11月至2020年4月济南地区312例6月龄内健康婴儿鼻咽拭子,增菌后,分离培养获得纯菌株;利用MALDI-TOF MS技术鉴定分离株到种置信水平;312例婴儿分为月龄≤2组、3月龄组、4月龄组和5月龄组,组间比较采用SPSS 22.0统计学软件,卡方或Fisher确切概率法统计分析。结果312例健康婴儿鼻咽拭子获得纯菌株741株,分属于25个属74种细菌。优势菌属为葡萄球菌属(31.6%)、棒杆菌属(30.8%)、链球菌属(18.9%)、莫拉菌属(8.6%)和狡诈球菌属(2.0%)。同时发现一些不常见菌种,例如缺陷乏氧菌、放射根瘤菌等。金黄色葡萄球菌检出率男婴(40.5%)高于女婴(26.4%),接近棒杆菌女婴(42.4%)高于男婴(27.4%)。4个年龄组中,假白喉棒杆菌检出率依次升高(10.8%、17.7%、18.9%、46.7%);肺炎链球菌检出率依次升高(2.2%、2.3%、4.1%、20.0%)。结论济南地区健康6月龄内婴儿鼻咽部微生态菌群以优势菌为主呈现多样化,MALDI-TOF MS是鉴定常见和不常见微生物菌种的快速、高通量技术手段。本研究可为济南地区儿童鼻咽部微生态研究提供有价值的样本库资料。Objective To explore the distribution characteristics of nasopharyngeal microflora in healthy infants under 6 months of age using matrix-assisted laser desorption ionization time of flight mass spectrometry(MALDI-TOF MS)so as to provide sample bank information for nasopharyngeal microecology research.Methods Nasopharyngeal swabs were collected from 312 healthy infants during Nov.2019 and April 2020 in Jinan.The pure strains were obtained after culturing and isolating.The bacterial strains were identified by MALDI-TOF MS to the species-level.The infants were divided into four age groups(≤2 months,3 months group,4 months group and 5 months group).SPSS 22.0 software was used for chi-square test or Fisher exact test between groups.Results A total of 741 bacterial strains were isolated,including 74 species of 25 genera of bacteria.The dominant genera were Staphylococcus(31.6%),Corynebacterium(30.8%),Streptococcus(18.9%),Moraxella(8.6%)and Dolosicoccus(2.0%).Some uncommon bacteria were also found,such as Abiotrophia defectiva,Rhizobium radiobacter,et al.The detection rate of Staphylococcus aureus was higher in male infants(40.5%)than in female infants(26.4%),while the detection rate of Corynebacterium propinquum was higher in female infants(42.4%)than in male infants(27.4%).In the four age groups,the detection rate of Corynebacterium pseudodiphtheriticum was increased successively(10.8%,17.7%,18.9%and 46.7%),as the same for Streptococcus pneumoniae(2.2%,2.3%,4.1%and 20.0%).Conclusion The diversity is mainly characterized by dominant bacteria in nasopharyngeal microflora for healthy infants under 6 months of age in Jinan,China.MALDI-TOF MS is a rapid and high-throughput technique for identifying common and uncommon bacterial species.The results can provide valuable reference for the study of nasopharyngeal microecology of children in Jinan.

关 键 词：鼻咽部 基质辅助激光解吸电离飞行时间质谱 微生态 婴儿 

分 类 号：R72[医药卫生—儿科]