聚腺苷酸二磷酸核糖聚合酶-1在子宫内膜异位症中的表达及意义  

Expression and significance of poly (ADP-ribose) polymerase-1 in endometriosis

作　　者：林雪艳 张灿灿[2] 田民乐 田永杰 LIN Xueyan;ZHANG Cancan;TIAN Minle;TIAN Yongjie(Department of Obstetrics and Gynecology,Shandong Provincial Hospital Affiliated to Shandong Frist Medical University,Jinan 250021,Shandong,China;Department of Gynecology,Shandong Provincial Maternal and Child Health Care Hospital,Cheeloo College of Medicine,Shandong University,Jinan 250014,Shandong,China;School of Basic Medicine,Shandong First Medical University,Jinan 250118,Shandong,China;Department of Obstetrics and Gynecology,Shandong Provincial Hospital,Cheeloo College of Medicine,Shandong University,Jinan 250021,Shandong,China)

机构地区：[1]山东第一医科大学附属省立医院妇产科,山东济南250021 [2]山东大学附属山东省妇幼保健院妇科,山东济南250014 [3]山东第一医科大学基础医学院,山东济南250118 [4]山东大学附属省立医院妇产科,山东济南250021

出　　处：《山东大学学报（医学版）》2022年第2期27-31,42,共6页Journal of Shandong University:Health Sciences

基　　金：山东省自然科学基金(ZR2019MH129);中华预防医学会科研资金支持项目(201815072)

摘　　要：目的检测聚腺苷酸二磷酸核糖聚合酶-1(PARP-1)在子宫内膜异位症患者在位内膜和异位内膜组织中的表达,并探讨PARP-1与E-cadeherin、vimentin和Snail在子宫内膜异位症患者内膜中表达的相关性。方法采用免疫组织化学SP法检测PARP-1、E-cadeherin、vimentin和Snail在子宫内膜异位症患者在位内膜、异位内膜、正常子宫在位内膜中的表达。采用Spearman方法分析PARP-1与E-cadeherin、vimentin和Snail之间表达的相关性。结果PARP-1、vimentin和Snail在子宫内膜异位症患者在位内膜及异位内膜中的表达比正常子宫在位内膜中的表达增高(P<0.001),E-cadherin在子宫内膜异位症患者在位内膜及异位内膜中的表达较正常子宫在位内膜表达降低(P<0.001)。在子宫内膜异位症患者在位内膜中,PARP-1的表达与E-cadherin呈负相关(r=-0.39),与vimentin和Snail的表达呈正相关(r分别为0.45和0.44);在子宫内膜异位症患者异位内膜中,PARP-1的表达与E-cadherin呈负相关(r=-0.41),与vimentin和Snail的表达呈正相关(r分别为0.48和0.56)。结论PARP-1在子宫内膜异位症在位内膜及异位内膜中表达增高,PARP-1可能参与了子宫内膜异位症的上皮间质转化过程。Objective To detect the expression of poly(ADP-ribose)polymerase-1(PARP-1)in eutopic and ectopic endometria of women with endometriosis,and to explore the correlation among PARP-1,E-cadherin,vimentin and Snail in eutopic and ectopic endometria of women with endometriosis.Methods The expressions of PARP-1,E-cadherin,Vimentin and Snail were detected with immunohistochemistry in eutopic and ectopic endometria of women with endometriosis,and in eutopic endometria of women without endometriosis.The correlation among their expressions was determined with Spearman analysis.Results The expressions of PARP-1,vimentin and Snail significantly increased in eutopic and ectopic endometria of women with endometriosis than in endometria of women without endometriosis(P<0.001),while the expression of E-cadherin significantly decreased(P<0.001).In eutopic endometria of endometriosis,the expression of PARP-1 was negatively correlated with E-cadherin(r=-0.39),but positively correlated with the expressions of vimentin and Snail(r=0.45 and 0.44,respectively);in ectopic endometria of endometriosis,the expression of PARP-1 was negatively correlated with E-cadherin(r=-0.41),but positively correlated with the expressions of vimentin and Snail(r=0.48 and 0.56,respectively).Conclusion PARP-1 is highly expressed in eutopic and ectopic endometria of women with endometriosis,PARP-1 may be involved in the epithelial mesenchymal transformation of endometriosis.

关 键 词：聚腺苷酸二磷酸核糖聚合酶-1 子宫内膜异位症 上皮-间质转化 E-CADHERIN蛋白 SNAIL蛋白 

分 类 号：R711.71[医药卫生—妇产科学]