“不可培养”微生物的分离培养方法研究进展  

Research progress in isolation and culture methods of"non-culturable"microorganisms

作　　者：赵俊天 张莹 ZHAO Juntian;ZHANG Ying(School of Life Science,Beijing Institute of Technology,Beijing,100081)

机构地区：[1]北京理工大学生命学院,北京100081

出　　处：《生命科学仪器》2023年第1期11-21,共11页Life Science Instruments

基　　金：国家自然科学基金项目(32270119)

摘　　要：近年来随着微生物学的发展,微生物资源挖掘成为热点问题。本文首先介绍了微生物纯培养在微生物学研究中的重要作用,以及自然界大多数微生物仍然无法在实验室中得到培养的现状;基于这一矛盾,详细介绍了近十年出现的新型微生物培养方法和装置并佐以实例;最后对现有微生物培养方法进行总结和展望,希望可以为未来微生物培养方法的创新和发展带来灵感,助力微生物资源的开发与利用。In recent years,with the development of microbiology,the exploration of microbial resources has been a topic of interest for researchers globally.This paper introduces the importance of obtaining pure culture of microorganisms in microbiological research,and the fact that most microorganisms in nature are still unable to be cultured in the laboratory.Based on this contradiction,this paper introduces in detail the new microbial culture methods and devices that have emerged in the past decade,their working and success.Finally,the existing microbial culture methods are compared,and some future prospects are presented in a quest to bring inspiration for the innovation and development of future microbial culture methods.This may result in a better exploration as well as utilization of different microbial resources.

关 键 词：微生物纯培养新方法 微生物种质资源 不可培养微生物 

分 类 号：Q93[生物学—微生物学]