地龙胶囊联合放疗对喉鳞癌裸鼠移植瘤血管生成的抑制作用  

Inhibitory Effect of Dilong Capsules Combined with Radiotherapy on Tumor Angiogenesis of Transplanted Laryngeal Dquamous Vell Varcinoma in Nude Mice

作　　者：张瑞芳 侯继崇 张玲娟 ZHANG Rui-Fang;HOU Ji-Chong;ZHANG Ling-Juan(Hengshui People’s Hospital,Hengshui 053000 Hebei,China;Hengshui Seventh People’s Hospital,Hengshui 053000 Hebei,China;Xingtai Third Hospital,Xingtai 054000 Hebei,China)

机构地区：[1]衡水市人民医院,河北衡水053000 [2]衡水市第七人民医院,河北衡水053000 [3]邢台市第三医院,河北邢台054000

出　　处：《广州中医药大学学报》2022年第5期1151-1155,共5页Journal of Guangzhou University of Traditional Chinese Medicine

摘　　要：【目的】观察地龙胶囊联合放疗对喉鳞癌裸鼠移植瘤血管生成的抑制作用。【方法】将浓度为1×10^(8)个/mL的人喉鳞癌细胞注射于裸鼠背部皮下构建喉鳞癌模型。将造模成功的喉鳞癌裸鼠分为模型组、放疗组、地龙胶囊组、联合组,每组10只。放疗组:用γ射线局部外照裸鼠移植瘤,共24 Gy,共12 d;地龙胶囊组:给予地龙胶囊混悬液50 mg·kg^(-1)·d^(-1)灌胃,连续干预2周;联合组:地龙胶囊联合放疗共同进行治疗,方法同地龙胶囊组和放疗组。模型组:给予等体积生理盐水灌胃。记录各组裸鼠移植瘤体质量与体积,免疫荧光法检测移植瘤血小板内皮细胞黏附分子1(PECAM-1/CD31)及微血管密度(MVD),免疫组织化学法检测移植瘤组织生存素表达,蛋白免疫印迹(Western Blot)检测移植瘤组织缺氧诱导因子1α(HIF-1α)蛋白表达。【结果】与模型组比较,放疗组、地龙胶囊组、联合组裸鼠瘤体质量和体积、MVD、生存素阳性细胞率、HIF-1α蛋白表达水平均降低(P<0.05);放疗组瘤体质量和体积、MVD、生存素阳性细胞率、HIF-1α蛋白表达水平与地龙胶囊组比较,均无显著性差异(P>0.05);联合组裸鼠瘤体质量和体积、MVD、生存素阳性细胞率、HIF-1α蛋白表达水平低于放疗组、地龙胶囊组(P<0.05)。【结论】地龙胶囊联合放疗可能通过抑制HIF-1α蛋白表达后抑制肿瘤血管生成,促进细胞凋亡,从而抑制喉鳞癌裸鼠移植瘤生长。Objective To observe the inhibitory effect of Dilong Capsules combined with radiotherapy on angiogenesis of transplanted laryngeal squamous cell carcinoma(LSCC)in nude mice.Methods The LSCC model was established by injecting human LSCC cells at the concentration of 1×10^(8)cells/mL into the subcutaneous area of the back of nude mice.The nude mice with LSCC were divided into model group,radiotherapy group,Dilong Capsules group and combination group,with 10 mice in each group.Radiotherapy group:the transplanted tumor of nude mice was locally and externally radiated withγray at total dose of 24 Gy for 12 days;Dilong Capsules group:the treatment of 50 mg·kg^(-1)·d^(-1)of suspension of Dilong Capsules was given intragastrically,and the intervention lasted for 2 continuous weeks;combination group:the treatment of Dilong Capsules combination with radiotherapy was given in the same way as Dilong Capsules group and radiotherapy group;model group:gavage with normal saline.The mass and volume of transplanted tumor were recorded at different time points in each group.Platelet endothelial cell adhesion molecule 1(PECAM-1/CD31)and microvascular density(MVD)of transplanted tumor were detected by immunofluorescence assay,the expression of survivin in transplanted tumor tissue was detected by immunohistochemistry,and the protein expression of hypoxia-inducible factor 1α(HIF-1α)was detected by Western Blot.Results Compared with the model group,mass and volume of tumor body,MVD,survivin positive cell ratio and HIF-1αprotein expression level of nude mice in radiotherapy group,Dilong Capsules group and combination group were decreased(P<0.05);there were no significant differences in mass and volume of tumor body,MVD,survivin positive cell rate and HIF-1αprotein expression between the radiotherapy group and Dilong Capsules group(P>0.05);the mass and volume of tumor body,MVD,survivin positive cell ratio and HIF-1αprotein expression level in combination group were lower than those in radiotherapy group and Dilong Capsules group

关 键 词：地龙胶囊 放疗 喉鳞癌 血管生成 生存素 缺氧诱导因子1Α 裸鼠 

分 类 号：R285.5[医药卫生—中药学]